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@INPROCEEDINGS{Rossini:190161,
author = {Rossini, Micol and Alberti, Giorgio and Bozzi, Emiliano and
Celesti, Marco and Cilia, Chiara and Cogliati, Sergio and
Colombo, Roberto and Julitta, Tommaso and Juszczak,
Radosław and Miglietta, Franco and Panigada, Cinzia and
Pinto, Francisco and Sakowska, Karolina and Schickling, Anke
and Schuettemeyer, Dirk and Stróżecki, Marcin and
Tudoroiu, Marin and Rascher, Uwe},
title = {{M}onitoring the evolution of plant photosynthetic
performances using ground sun-induced fluorescence
measurements},
reportid = {FZJ-2015-03092},
year = {2015},
abstract = {Plants subjected to biotic or abiotic stress factors can
respond with adjustments in their biochemical and
physiological processes. These adjustments are often
accompanied by changes in reflectance, transmittance, and
absorbance at leaf and canopy level.This contribution aims
to understand how optical signals linked to plant physiology
changes after the application of two different chemical
agents: an herbicide blocking the electron-transfer
mechanisms in the photosynthetic apparatus (Chlortoluron)
and an anti-transpirant chemical known to reduce plant
transpiration (Vapor Gard) by sealing the stomata. The
chemicals have been applied on 9 grass plots with different
concentrations. Three plots have been kept not-treated and
used as control. Canopy high resolution spectral
measurements and CO2 fluxes have been collected daily on the
same sampling area in each plot for the entire duration of
the experiment to monitor the temporal evolution of the
stress effects.Spectral measurements have been used to
estimate the sun-induced chlorophyll fluorescence in both
the red (SIF687) and far-red region (SIF760), the
Photochemical Reflectance Index (PRI) linked to the
xanthophyll-related heat dissipation and several traditional
vegetation indices related to canopy greenness and
chlorophyll concentration. SIF687 and SIF760 were estimated
with spectral fitting methods spectrally modeling the
radiance collected with very high resolution spectrometers
in the oxygen absorption O2-A and O2-B bands.The applied
treatments induced a variation of plant photosynthetic
functioning modulated according to the level of herbicide
concentration. Both SIF687 and SIF760 measured in
Chlortoluron-treated grass plots were significantly higher
than in the control plots. The highest dose caused
fluorescence values to double in less than 3 hours while the
reflectance signal at the same time was not affected
confirming that the increase in fluorescence emission was
only related to variations in the plant functional status
not associated to changes in pigment content and
composition. F687 and F760 values of treated plots decreased
steeply in the following days. Fluorescence decline was
accompanied by a decrease in chlorophyll content. Grass
photosynthesis began to decline immediately after the
herbicide application and continued in the following days.
This result implies that fluorescence was negatively
correlated to plant photosynthesis in an early phase of
stress reaction and then the relation became positive in the
following days. Thus the translation of fluorescence values
to photosynthesis is not straightforward in this experiment.
Lower doses affected fluorescence signal similarly but with
a different temporal dynamic in both the initial rise and
the recovery. Chlortoluron also caused an initial increase
of PRI values followed by a gradual decline associated to
the degradation of the pigment pool. The application of the
anti-transpirant agent only slightly affected fluorescence
emission and PRI probably because the photosynthetic system
was not directly compromised.Sun-induced chlorophyll
fluorescence allowed to monitor the temporal dynamics of
plants’ functioning and recovery after the application of
temporarily blocking photosynthesis agents. Further studies
are ongoing to better understand the effects of stress on
the fluorescence signal and the link to heat dissipation and
photosynthesis.},
month = {Apr},
date = {2015-04-14},
organization = {9th EARSel Imaging Spectroscopy
Workshop, Luxembourg (Luxembourg), 14
Apr 2015 - 16 Apr 2015},
subtyp = {After Call},
cin = {IBG-2},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582)},
pid = {G:(DE-HGF)POF3-582},
typ = {PUB:(DE-HGF)6},
url = {https://juser.fz-juelich.de/record/190161},
}