%0 Journal Article
%A Galkin, V.E.
%A Orlova, A.
%A Kudryashov, D.S.
%A Solodukhin, A.
%A Reisler, E.
%A Schröder, G.F.
%A Egelman, E.H.
%T Remodeling of actin filaments by ADF/cofilin proteins
%J Proceedings of the National Academy of Sciences of the United States of America
%V 108
%@ 0027-8424
%C Washington, DC
%I Academy
%M PreJuSER-19044
%P 20568 - 20572
%D 2011
%Z This work was supported by National Institutes of Health Grants GM081303 (E. H. E.) and GM077190 (E.R.).
%X Cofilin/ADF proteins play key roles in the dynamics of actin, one of the most abundant and highly conserved eukaryotic proteins. We used cryoelectron microscopy to generate a 9-Å resolution three-dimensional reconstruction of cofilin-decorated actin filaments, the highest resolution achieved for a complex of F-actin with an actin-binding protein. We show that the cofilin-induced change in the filament twist is due to a unique conformation of the actin molecule unrelated to any previously observed state. The changes between the actin protomer in naked F-actin and in the actin-cofilin filament are greater than the conformational changes between G- and F-actin. Our results show the structural plasticity of actin, suggest that other actin-binding proteins may also induce large but different conformational changes, and show that F-actin cannot be described by a single molecular model.
%K Actin Depolymerizing Factors: chemistry
%K Actins: chemistry
%K Cofilin 2: chemistry
%K Cryoelectron Microscopy: methods
%K Cytoskeleton: chemistry
%K Gene Library
%K Humans
%K Microscopy, Electron: methods
%K Models, Molecular
%K Molecular Conformation
%K Muscle, Skeletal: metabolism
%K Polymers: chemistry
%K Protein Conformation
%K Protein Structure, Secondary
%K Actin Depolymerizing Factors (NLM Chemicals)
%K Actins (NLM Chemicals)
%K Cofilin 2 (NLM Chemicals)
%K Polymers (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:22158895
%2 pmc:PMC3251117
%U <Go to ISI:>//WOS:000298289400064
%R 10.1073/pnas.1110109108
%U https://juser.fz-juelich.de/record/19044