% IMPORTANT: The following is UTF-8 encoded. This means that in the presence % of non-ASCII characters, it will not work with BibTeX 0.99 or older. % Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or % “biber”. @ARTICLE{Galkin:19044, author = {Galkin, V.E. and Orlova, A. and Kudryashov, D.S. and Solodukhin, A. and Reisler, E. and Schröder, G.F. and Egelman, E.H.}, title = {{R}emodeling of actin filaments by {ADF}/cofilin proteins}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {108}, issn = {0027-8424}, address = {Washington, DC}, publisher = {Academy}, reportid = {PreJuSER-19044}, pages = {20568 - 20572}, year = {2011}, note = {This work was supported by National Institutes of Health Grants GM081303 (E. H. E.) and GM077190 (E.R.).}, abstract = {Cofilin/ADF proteins play key roles in the dynamics of actin, one of the most abundant and highly conserved eukaryotic proteins. We used cryoelectron microscopy to generate a 9-Å resolution three-dimensional reconstruction of cofilin-decorated actin filaments, the highest resolution achieved for a complex of F-actin with an actin-binding protein. We show that the cofilin-induced change in the filament twist is due to a unique conformation of the actin molecule unrelated to any previously observed state. The changes between the actin protomer in naked F-actin and in the actin-cofilin filament are greater than the conformational changes between G- and F-actin. Our results show the structural plasticity of actin, suggest that other actin-binding proteins may also induce large but different conformational changes, and show that F-actin cannot be described by a single molecular model.}, keywords = {Actin Depolymerizing Factors: chemistry / Actins: chemistry / Cofilin 2: chemistry / Cryoelectron Microscopy: methods / Cytoskeleton: chemistry / Gene Library / Humans / Microscopy, Electron: methods / Models, Molecular / Molecular Conformation / Muscle, Skeletal: metabolism / Polymers: chemistry / Protein Conformation / Protein Structure, Secondary / Actin Depolymerizing Factors (NLM Chemicals) / Actins (NLM Chemicals) / Cofilin 2 (NLM Chemicals) / Polymers (NLM Chemicals) / J (WoSType)}, cin = {ICS-6}, ddc = {000}, cid = {I:(DE-Juel1)ICS-6-20110106}, pnm = {Funktion und Dysfunktion des Nervensystems / BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung}, pid = {G:(DE-Juel1)FUEK409 / G:(DE-Juel1)FUEK505}, shelfmark = {Multidisciplinary Sciences}, typ = {PUB:(DE-HGF)16}, pubmed = {pmid:22158895}, pmc = {pmc:PMC3251117}, UT = {WOS:000298289400064}, doi = {10.1073/pnas.1110109108}, url = {https://juser.fz-juelich.de/record/19044}, }