TY - JOUR
AU - Atkinson, J.
AU - Kapralov, A.A.
AU - Yanamala, N.
AU - Tyurina, Y.Y.
AU - Amoscato, A.A.
AU - Pearce, L.
AU - Peterson, J.
AU - Huang, Z.
AU - Jiang, J.
AU - Samhan-Arias, A.K.
AU - Maeda, A.
AU - Feng, W.
AU - Wasserloos, K.
AU - Belikova, N.A.
AU - Tyurin, V.A.
AU - Wang, H.
AU - Fletcher, J.
AU - Wang, Y.
AU - Vlasova, Il.
AU - Klein-Seetharaman, J.
AU - Stoyanovsky, D.A.
AU - Bayir, H.
AU - Pitt, B.R.
AU - Epperly, M.W.
AU - Greenberger, J.S.
AU - Kagan, V.E.
TI - A mitochondrial-targeted inhibitor of cytochrorme c peroxidase mitigates radiation-induced death
JO - Nature Communications
VL - 2
SN - 2041-1723
CY - London
PB - Nature Publishing Group
M1 - PreJuSER-19623
SP - 497
PY - 2011
AB - The risk of radionuclide release in terrorist acts or exposure of healthy tissue during radiotherapy demand potent radioprotectants/radiomitigators. Ionizing radiation induces cell death by initiating the selective peroxidation of cardiolipin in mitochondria by the peroxidase activity of its complex with cytochrome c leading to release of haemoprotein into the cytosol and commitment to the apoptotic program. Here we design and synthesize mitochondria-targeted triphenylphosphonium-conjugated imidazole-substituted oleic and stearic acids that blocked peroxidase activity of cytochrome c/cardiolipin complex by specifically binding to its haem-iron. We show that both compounds inhibit pro-apoptotic oxidative events, suppress cyt c release, prevent cell death, and protect mice against lethal doses of irradiation. Significant radioprotective/radiomitigative effects of imidazole-substituted oleic acid are observed after pretreatment of mice from 1 h before through 24 h after the irradiation.
KW - Animals
KW - Cell Death: drug effects
KW - Cell Death: radiation effects
KW - Cytochrome-c Peroxidase: antagonists & inhibitors
KW - Electron Spin Resonance Spectroscopy
KW - Enzyme Inhibitors: chemistry
KW - Enzyme Inhibitors: pharmacology
KW - Female
KW - Mice
KW - Mice, Inbred C57BL
KW - Mitochondria: drug effects
KW - Mitochondria: enzymology
KW - Models, Molecular
KW - Molecular Dynamics Simulation
KW - Radiation-Protective Agents: chemistry
KW - Radiation-Protective Agents: pharmacology
KW - Enzyme Inhibitors (NLM Chemicals)
KW - Radiation-Protective Agents (NLM Chemicals)
KW - Cytochrome-c Peroxidase (NLM Chemicals)
KW - J (WoSType)
LB - PUB:(DE-HGF)16
C6 - pmid:21988913
UR - <Go to ISI:>//WOS:000296787300010
DO - DOI:10.1038/ncomms1499
UR - https://juser.fz-juelich.de/record/19623
ER -