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@ARTICLE{Pick:19648,
author = {Pick, T.R. and Bräutigam, A. and Schlüter, U. and Denton,
A.K. and Colmsee, C. and Scholz, U. and Fahnenstich, H. and
Pieruschka, R. and Rascher, U. and Sonnewald, U. and Weber,
A.P.M.},
title = {{S}ystems {A}nalysis of a {M}aize {L}eaf {D}evelopmental
{G}radient {R}edefines the {C}urrent {C}4 {M}odel and
{P}rovides {C}anditates for {R}egulation},
journal = {THE PLANT CELL ONLINE},
volume = {23},
issn = {1040-4651},
address = {Rockville, Md.},
publisher = {Soc.},
reportid = {PreJuSER-19648},
pages = {1 - 13},
year = {2012},
note = {This study was supported by a grant of the German Federal
Ministry of Education and Research (BioEnergy 2021, OPTIMAS)
and grants from the German Research Foundation (IRTG 1525
and FOR 1186 PROMICS to A.P.M.W.). We thank Katrin L. Weber
for support with GC-MS analyses.},
abstract = {We systematically analyzed a developmental gradient of the
third maize (Zea mays) leaf from the point of emergence into
the light to the tip in 10 continuous leaf slices to study
organ development and physiological and biochemical
functions. Transcriptome analysis, oxygen sensitivity of
photosynthesis, and photosynthetic rate measurements showed
that the maize leaf undergoes a sink-to-source transition
without an intermediate phase of C(3) photosynthesis or
operation of a photorespiratory carbon pump. Metabolome and
transcriptome analysis, chlorophyll and protein
measurements, as well as dry weight determination, showed
continuous gradients for all analyzed items. The absence of
binary on-off switches and regulons pointed to a
morphogradient along the leaf as the determining factor of
developmental stage. Analysis of transcription factors for
differential expression along the leaf gradient defined a
list of putative regulators orchestrating the sink-to-source
transition and establishment of C(4) photosynthesis.
Finally, transcriptome and metabolome analysis, as well as
enzyme activity measurements, and absolute quantification of
selected metabolites revised the current model of maize C(4)
photosynthesis. All data sets are included within the
publication to serve as a resource for maize leaf systems
biology.},
keywords = {Chlorophyll: analysis / Chlorophyll: chemistry / Cluster
Analysis / Enzyme Activation / Gene Expression Regulation,
Plant / Genes, Plant / Light / Malates: chemistry /
Metabolome / Oxygen: chemistry / Photosynthesis / Plant
Leaves: chemistry / Plant Leaves: genetics / Plant Leaves:
physiology / Plant Proteins: chemistry / Plant Proteins:
genetics / Pyruvic Acid: chemistry / Transcription Factors:
chemistry / Transcription Factors: genetics / Transcription,
Genetic / Transcriptome / Zea mays: chemistry / Zea mays:
genetics / Zea mays: physiology / Malates (NLM Chemicals) /
Plant Proteins (NLM Chemicals) / Transcription Factors (NLM
Chemicals) / Pyruvic Acid (NLM Chemicals) / Chlorophyll (NLM
Chemicals) / malic acid (NLM Chemicals) / Oxygen (NLM
Chemicals) / J (WoSType)},
cin = {IBG-2},
ddc = {570},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {Terrestrische Umwelt},
pid = {G:(DE-Juel1)FUEK407},
shelfmark = {Biochemistry $\&$ Molecular Biology / Plant Sciences / Cell
Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:22186372},
pmc = {pmc:PMC3269860},
UT = {WOS:000299677700008},
doi = {10.1105/tpc.111.090324},
url = {https://juser.fz-juelich.de/record/19648},
}