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000019667 084__ $$2WoS$$aPhysics, Atomic, Molecular & Chemical
000019667 1001_ $$0P:(DE-HGF)0$$aGögelein, C.$$b0
000019667 245__ $$aEffect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments
000019667 260__ $$aMelville, NY$$bAmerican Institute of Physics$$c2012
000019667 300__ $$a015102
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000019667 440_0 $$03145$$aJournal of Chemical Physics$$v136$$x0021-9606$$y1
000019667 500__ $$aWe thank the International Helmholtz Research School of Biophysics and Soft Matter (IHRS BioSoft) (IHRS BioSoft) and the Deutsche Forschungsgemeinschaft (DFG) for financial support.
000019667 520__ $$aSalt, glycerol, and dimethyl sulfoxide (DMSO) are used to modify the properties of protein solutions. We experimentally determined the effect of these additives on the phase behavior of lysozyme solutions. Upon the addition of glycerol and DMSO, the fluid-solid transition and the gas-liquid coexistence curve (binodal) shift to lower temperatures and the gap between them increases. The experimentally observed trends are consistent with our theoretical predictions based on the thermodynamic perturbation theory and the Derjaguin-Landau-Verwey-Overbeek model for the lysozyme-lysozyme pair interactions. The values of the parameters describing the interactions, namely the refractive indices, dielectric constants, Hamaker constant and cut-off length, are extracted from literature or are experimentally determined by independent experiments, including static light scattering, to determine the second virial coefficient. We observe that both, glycerol and DMSO, render the potential more repulsive, while sodium chloride reduces the repulsion.
000019667 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
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000019667 650_2 $$2MeSH$$aDimethyl Sulfoxide: chemistry
000019667 650_2 $$2MeSH$$aGlycerol: chemistry
000019667 650_2 $$2MeSH$$aMuramidase: chemistry
000019667 650_2 $$2MeSH$$aMuramidase: metabolism
000019667 650_2 $$2MeSH$$aProtein Binding
000019667 650_2 $$2MeSH$$aQuantum Theory
000019667 650_2 $$2MeSH$$aSodium Chloride: chemistry
000019667 650_2 $$2MeSH$$aThermodynamics
000019667 650_7 $$056-81-5$$2NLM Chemicals$$aGlycerol
000019667 650_7 $$067-68-5$$2NLM Chemicals$$aDimethyl Sulfoxide
000019667 650_7 $$07647-14-5$$2NLM Chemicals$$aSodium Chloride
000019667 650_7 $$0EC 3.2.1.17$$2NLM Chemicals$$aMuramidase
000019667 650_7 $$2WoSType$$aJ
000019667 65320 $$2Author$$aadditives
000019667 65320 $$2Author$$abiochemistry
000019667 65320 $$2Author$$aenzymes
000019667 65320 $$2Author$$aequations of state
000019667 65320 $$2Author$$alight scattering
000019667 65320 $$2Author$$amolecular biophysics
000019667 65320 $$2Author$$apermittivity
000019667 65320 $$2Author$$aperturbation theory
000019667 65320 $$2Author$$aphysiological models
000019667 65320 $$2Author$$arefractive index
000019667 7001_ $$0P:(DE-HGF)0$$aWagner, D.$$b1
000019667 7001_ $$0P:(DE-HGF)0$$aCardinaux, F.$$b2
000019667 7001_ $$0P:(DE-Juel1)130858$$aNägele, G.$$b3$$uFZJ
000019667 7001_ $$0P:(DE-HGF)0$$aEgelhaaf, S.U.$$b4
000019667 773__ $$0PERI:(DE-600)1473050-9$$a10.1063/1.3673442$$gVol. 136, p. 015102$$p015102$$q136<015102$$tThe @journal of chemical physics$$v136$$x0021-9606$$y2012
000019667 8567_ $$uhttp://dx.doi.org/10.1063/1.3673442
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