Home > Publications database > Effect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments > print

001     19667
005     20240619083343.0
024 7 _ |2 pmid
|a pmid:22239802
024 7 _ |2 DOI
|a 10.1063/1.3673442
024 7 _ |2 WOS
|a WOS:000298967200040
024 7 _ |a 2128/4639
|2 Handle
037 _ _ |a PreJuSER-19667
041 _ _ |a eng
082 _ _ |a 540
084 _ _ |2 WoS
|a Physics, Atomic, Molecular & Chemical
100 1 _ |a Gögelein, C.
|b 0
|0 P:(DE-HGF)0
245 _ _ |a Effect of glycerol and dimethyl sulfoxide on the phase behavior of lysozyme: Theory and experiments
260 _ _ |a Melville, NY
|b American Institute of Physics
|c 2012
300 _ _ |a 015102
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Journal of Chemical Physics
|x 0021-9606
|0 3145
|y 1
|v 136
500 _ _ |a We thank the International Helmholtz Research School of Biophysics and Soft Matter (IHRS BioSoft) (IHRS BioSoft) and the Deutsche Forschungsgemeinschaft (DFG) for financial support.
520 _ _ |a Salt, glycerol, and dimethyl sulfoxide (DMSO) are used to modify the properties of protein solutions. We experimentally determined the effect of these additives on the phase behavior of lysozyme solutions. Upon the addition of glycerol and DMSO, the fluid-solid transition and the gas-liquid coexistence curve (binodal) shift to lower temperatures and the gap between them increases. The experimentally observed trends are consistent with our theoretical predictions based on the thermodynamic perturbation theory and the Derjaguin-Landau-Verwey-Overbeek model for the lysozyme-lysozyme pair interactions. The values of the parameters describing the interactions, namely the refractive indices, dielectric constants, Hamaker constant and cut-off length, are extracted from literature or are experimentally determined by independent experiments, including static light scattering, to determine the second virial coefficient. We observe that both, glycerol and DMSO, render the potential more repulsive, while sodium chloride reduces the repulsion.
536 _ _ |a BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
|c P45
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK505
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Dimethyl Sulfoxide: chemistry
650 _ 2 |2 MeSH
|a Glycerol: chemistry
650 _ 2 |2 MeSH
|a Muramidase: chemistry
650 _ 2 |2 MeSH
|a Muramidase: metabolism
650 _ 2 |2 MeSH
|a Protein Binding
650 _ 2 |2 MeSH
|a Quantum Theory
650 _ 2 |2 MeSH
|a Sodium Chloride: chemistry
650 _ 2 |2 MeSH
|a Thermodynamics
650 _ 7 |0 56-81-5
|2 NLM Chemicals
|a Glycerol
650 _ 7 |0 67-68-5
|2 NLM Chemicals
|a Dimethyl Sulfoxide
650 _ 7 |0 7647-14-5
|2 NLM Chemicals
|a Sodium Chloride
650 _ 7 |0 EC 3.2.1.17
|2 NLM Chemicals
|a Muramidase
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a additives
653 2 0 |2 Author
|a biochemistry
653 2 0 |2 Author
|a enzymes
653 2 0 |2 Author
|a equations of state
653 2 0 |2 Author
|a light scattering
653 2 0 |2 Author
|a molecular biophysics
653 2 0 |2 Author
|a permittivity
653 2 0 |2 Author
|a perturbation theory
653 2 0 |2 Author
|a physiological models
653 2 0 |2 Author
|a refractive index
700 1 _ |a Wagner, D.
|b 1
|0 P:(DE-HGF)0
700 1 _ |a Cardinaux, F.
|b 2
|0 P:(DE-HGF)0
700 1 _ |a Nägele, G.
|b 3
|u FZJ
|0 P:(DE-Juel1)130858
700 1 _ |a Egelhaaf, S.U.
|b 4
|0 P:(DE-HGF)0
773 _ _ |a 10.1063/1.3673442
|g Vol. 136, p. 015102
|p 015102
|q 136<015102
|0 PERI:(DE-600)1473050-9
|t The @journal of chemical physics
|v 136
|y 2012
|x 0021-9606
856 7 _ |u http://dx.doi.org/10.1063/1.3673442
856 4 _ |u https://juser.fz-juelich.de/record/19667/files/4639.pdf
|y OpenAccess
856 4 _ |u https://juser.fz-juelich.de/record/19667/files/FZJ-19667.pdf
|y Published under German "Allianz" Licensing conditions on 2012-01-04. Available in OpenAccess from 2012-01-04
|z Published final document.
856 4 _ |u https://juser.fz-juelich.de/record/19667/files/4639.jpg?subformat=icon-1440
|x icon-1440
|y OpenAccess
856 4 _ |u https://juser.fz-juelich.de/record/19667/files/4639.jpg?subformat=icon-180
|x icon-180
|y OpenAccess
856 4 _ |u https://juser.fz-juelich.de/record/19667/files/4639.jpg?subformat=icon-640
|x icon-640
|y OpenAccess
856 4 _ |u https://juser.fz-juelich.de/record/19667/files/FZJ-19667.jpg?subformat=icon-1440
|x icon-1440
856 4 _ |u https://juser.fz-juelich.de/record/19667/files/FZJ-19667.jpg?subformat=icon-180
|x icon-180
856 4 _ |u https://juser.fz-juelich.de/record/19667/files/FZJ-19667.jpg?subformat=icon-640
|x icon-640
909 C O |o oai:juser.fz-juelich.de:19667
|p openaire
|p open_access
|p driver
|p VDB
|p dnbdelivery
913 1 _ |b Schlüsseltechnologien
|k P45
|l Biologische Informationsverarbeitung
|1 G:(DE-HGF)POF2-450
|0 G:(DE-Juel1)FUEK505
|2 G:(DE-HGF)POF2-400
|v BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
|x 0
913 2 _ |a DE-HGF
|b Key Technologies
|l BioSoft Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences
|1 G:(DE-HGF)POF3-550
|0 G:(DE-HGF)POF3-551
|2 G:(DE-HGF)POF3-500
|v Functional Macromolecules and Complexes
|x 0
914 1 _ |y 2012
915 _ _ |a JCR/ISI refereed
|0 StatID:(DE-HGF)0010
|2 StatID
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
915 _ _ |a WoS
|0 StatID:(DE-HGF)0110
|2 StatID
|b Science Citation Index
915 _ _ |a WoS
|0 StatID:(DE-HGF)0111
|2 StatID
|b Science Citation Index Expanded
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Thomson Reuters Master Journal List
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0310
|2 StatID
|b NCBI Molecular Biology Database
915 _ _ |a Allianz-Lizenz / DFG
|0 StatID:(DE-HGF)0400
|2 StatID
915 _ _ |a Nationallizenz
|0 StatID:(DE-HGF)0420
|2 StatID
915 _ _ |a OpenAccess
|0 StatID:(DE-HGF)0510
|2 StatID
915 _ _ |a Allianz-OA
|0 StatID:(DE-HGF)0520
|2 StatID
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1020
|2 StatID
|b Current Contents - Social and Behavioral Sciences
920 1 _ |k ICS-3
|l Weiche Materie
|g ICS
|0 I:(DE-Juel1)ICS-3-20110106
|x 0
970 _ _ |a VDB:(DE-Juel1)134559
980 1 _ |a FullTexts
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a journal
980 _ _ |a I:(DE-Juel1)ICS-3-20110106
980 _ _ |a UNRESTRICTED
980 _ _ |a JUWEL
980 _ _ |a JUWEL
980 _ _ |a FullTexts

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21