TY  - JOUR
AU  - Gould, N.
AU  - Thorpe, M. R.
AU  - Pritchard, J.
AU  - Christeller, J.I.
AU  - Williams, L.E.
AU  - Roeb, G.
AU  - Schurr, U.
AU  - Minchin, P. E. H.
TI  - AtSUC2 has a role for sucrose retrieval along the phloem pathway: Evidence from carbon-11 tracer studies
JO  - Plant science
VL  - 188-189
SN  - 0168-9452
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PreJuSER-19781
SP  - 97 - 101
PY  - 2012
N1  - This work began in New Zealand using the <SUP>11</SUP>C facility for plant science maintained by the Geological and Nuclear Sciences Crown Research Institute in Lower Hutt, and finished in Germany at the Phytosphere Institute (now Plant Sciences) in the Forschungszentrum Julich. Mr. Chris Purcell in New Zealand, and Mr. Marko Dautzeuberg in Germany, provided exemplary support at all hours. Dr. Laurence Gatehouse and Helen Boldingh (Plant & Food Research) provided valuable comments on the manuscript. Financial support was provided by the New Zealand Marsden Fund (Contract Number UOW003) and by the Forschungszentrum Julich. Peter Minchin and Michael Thorpe wish to thank colleagues at both locations for their support and encouragement during a difficult period when their employment was terminated and the <SUP>11</SUP>C-facility in New Zealand was closed.
AB  - The location of the phloem within a plant, and its vulnerability to disruption, make it a difficult tissue to study and therefore non-invasive studies of phloem functionality are important. Here we compare, phloem transport, measured non-invasively, in wild type Arabidopsis thaliana, and transposon-insertion mutants for AtSUC1 or AtSUC2, giving in vivo information on the importance of these sucrose transporters for phloem transport. The suc2 mutant showed an increase in both phloem leakage and transport time, consistent with reduced sucrose uptake into both transport and collection phloem. The results are consistent with the AtSUC2 transporter being important for retrieval of leaked sucrose in the transport phloem of Arabidopsis. There was no difference in phloem transport properties between the wild type and the suc1 mutants, implying that the AtSUC1 transporter does not play a significant role within the transport phloem of Arabidopsis under the conditions of our study.
KW  - Arabidopsis: genetics
KW  - Arabidopsis: metabolism
KW  - Biological Transport
KW  - Carbon: metabolism
KW  - Carbon Radioisotopes: diagnostic use
KW  - Gene Expression Regulation, Plant: genetics
KW  - Gene Knockout Techniques
KW  - Membrane Transport Proteins: genetics
KW  - Membrane Transport Proteins: metabolism
KW  - Mutagenesis, Insertional
KW  - Phloem: metabolism
KW  - Plant Proteins: genetics
KW  - Plant Proteins: metabolism
KW  - Sucrose: metabolism
KW  - Carbon Radioisotopes (NLM Chemicals)
KW  - Membrane Transport Proteins (NLM Chemicals)
KW  - Plant Proteins (NLM Chemicals)
KW  - sucrose transport protein, plant (NLM Chemicals)
KW  - Sucrose (NLM Chemicals)
KW  - Carbon (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:22525249
UR  - <Go to ISI:>//WOS:000304214800012
DO  - DOI:10.1016/j.plantsci.2011.12.018
UR  - https://juser.fz-juelich.de/record/19781
ER  -