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@ARTICLE{Gould:19781,
author = {Gould, N. and Thorpe, M. R. and Pritchard, J. and
Christeller, J.I. and Williams, L.E. and Roeb, G. and
Schurr, U. and Minchin, P. E. H.},
title = {{A}t{SUC}2 has a role for sucrose retrieval along the
phloem pathway: {E}vidence from carbon-11 tracer studies},
journal = {Plant science},
volume = {188-189},
issn = {0168-9452},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {PreJuSER-19781},
pages = {97 - 101},
year = {2012},
note = {This work began in New Zealand using the <SUP>11</SUP>C
facility for plant science maintained by the Geological and
Nuclear Sciences Crown Research Institute in Lower Hutt, and
finished in Germany at the Phytosphere Institute (now Plant
Sciences) in the Forschungszentrum Julich. Mr. Chris Purcell
in New Zealand, and Mr. Marko Dautzeuberg in Germany,
provided exemplary support at all hours. Dr. Laurence
Gatehouse and Helen Boldingh (Plant $\&$ Food Research)
provided valuable comments on the manuscript. Financial
support was provided by the New Zealand Marsden Fund
(Contract Number UOW003) and by the Forschungszentrum
Julich. Peter Minchin and Michael Thorpe wish to thank
colleagues at both locations for their support and
encouragement during a difficult period when their
employment was terminated and the <SUP>11</SUP>C-facility in
New Zealand was closed.},
abstract = {The location of the phloem within a plant, and its
vulnerability to disruption, make it a difficult tissue to
study and therefore non-invasive studies of phloem
functionality are important. Here we compare, phloem
transport, measured non-invasively, in wild type Arabidopsis
thaliana, and transposon-insertion mutants for AtSUC1 or
AtSUC2, giving in vivo information on the importance of
these sucrose transporters for phloem transport. The suc2
mutant showed an increase in both phloem leakage and
transport time, consistent with reduced sucrose uptake into
both transport and collection phloem. The results are
consistent with the AtSUC2 transporter being important for
retrieval of leaked sucrose in the transport phloem of
Arabidopsis. There was no difference in phloem transport
properties between the wild type and the suc1 mutants,
implying that the AtSUC1 transporter does not play a
significant role within the transport phloem of Arabidopsis
under the conditions of our study.},
keywords = {Arabidopsis: genetics / Arabidopsis: metabolism /
Biological Transport / Carbon: metabolism / Carbon
Radioisotopes: diagnostic use / Gene Expression Regulation,
Plant: genetics / Gene Knockout Techniques / Membrane
Transport Proteins: genetics / Membrane Transport Proteins:
metabolism / Mutagenesis, Insertional / Phloem: metabolism /
Plant Proteins: genetics / Plant Proteins: metabolism /
Sucrose: metabolism / Carbon Radioisotopes (NLM Chemicals) /
Membrane Transport Proteins (NLM Chemicals) / Plant Proteins
(NLM Chemicals) / sucrose transport protein, plant (NLM
Chemicals) / Sucrose (NLM Chemicals) / Carbon (NLM
Chemicals) / J (WoSType)},
cin = {IBG-2},
ddc = {580},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {Terrestrische Umwelt},
pid = {G:(DE-Juel1)FUEK407},
shelfmark = {Biochemistry $\&$ Molecular Biology / Plant Sciences},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:22525249},
UT = {WOS:000304214800012},
doi = {10.1016/j.plantsci.2011.12.018},
url = {https://juser.fz-juelich.de/record/19781},
}