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024 7 _ |2 pmid
|a pmid:22525249
024 7 _ |2 DOI
|a 10.1016/j.plantsci.2011.12.018
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|a WOS:000304214800012
037 _ _ |a PreJuSER-19781
041 _ _ |a eng
082 _ _ |a 580
084 _ _ |2 WoS
|a Biochemistry & Molecular Biology
084 _ _ |2 WoS
|a Plant Sciences
100 1 _ |0 P:(DE-HGF)0
|a Gould, N.
|b 0
245 _ _ |a AtSUC2 has a role for sucrose retrieval along the phloem pathway: Evidence from carbon-11 tracer studies
260 _ _ |a Amsterdam [u.a.]
|b Elsevier Science
|c 2012
300 _ _ |a 97 - 101
336 7 _ |a Journal Article
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336 7 _ |a article
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440 _ 0 |0 25904
|a Plant Science
|v 188 - 189
500 _ _ |a This work began in New Zealand using the 11C facility for plant science maintained by the Geological and Nuclear Sciences Crown Research Institute in Lower Hutt, and finished in Germany at the Phytosphere Institute (now Plant Sciences) in the Forschungszentrum Julich. Mr. Chris Purcell in New Zealand, and Mr. Marko Dautzeuberg in Germany, provided exemplary support at all hours. Dr. Laurence Gatehouse and Helen Boldingh (Plant & Food Research) provided valuable comments on the manuscript. Financial support was provided by the New Zealand Marsden Fund (Contract Number UOW003) and by the Forschungszentrum Julich. Peter Minchin and Michael Thorpe wish to thank colleagues at both locations for their support and encouragement during a difficult period when their employment was terminated and the 11C-facility in New Zealand was closed.
520 _ _ |a The location of the phloem within a plant, and its vulnerability to disruption, make it a difficult tissue to study and therefore non-invasive studies of phloem functionality are important. Here we compare, phloem transport, measured non-invasively, in wild type Arabidopsis thaliana, and transposon-insertion mutants for AtSUC1 or AtSUC2, giving in vivo information on the importance of these sucrose transporters for phloem transport. The suc2 mutant showed an increase in both phloem leakage and transport time, consistent with reduced sucrose uptake into both transport and collection phloem. The results are consistent with the AtSUC2 transporter being important for retrieval of leaked sucrose in the transport phloem of Arabidopsis. There was no difference in phloem transport properties between the wild type and the suc1 mutants, implying that the AtSUC1 transporter does not play a significant role within the transport phloem of Arabidopsis under the conditions of our study.
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Arabidopsis: genetics
650 _ 2 |2 MeSH
|a Arabidopsis: metabolism
650 _ 2 |2 MeSH
|a Biological Transport
650 _ 2 |2 MeSH
|a Carbon: metabolism
650 _ 2 |2 MeSH
|a Carbon Radioisotopes: diagnostic use
650 _ 2 |2 MeSH
|a Gene Expression Regulation, Plant: genetics
650 _ 2 |2 MeSH
|a Gene Knockout Techniques
650 _ 2 |2 MeSH
|a Membrane Transport Proteins: genetics
650 _ 2 |2 MeSH
|a Membrane Transport Proteins: metabolism
650 _ 2 |2 MeSH
|a Mutagenesis, Insertional
650 _ 2 |2 MeSH
|a Phloem: metabolism
650 _ 2 |2 MeSH
|a Plant Proteins: genetics
650 _ 2 |2 MeSH
|a Plant Proteins: metabolism
650 _ 2 |2 MeSH
|a Sucrose: metabolism
650 _ 7 |0 0
|2 NLM Chemicals
|a Carbon Radioisotopes
650 _ 7 |0 0
|2 NLM Chemicals
|a Membrane Transport Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Plant Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a sucrose transport protein, plant
650 _ 7 |0 57-50-1
|2 NLM Chemicals
|a Sucrose
650 _ 7 |0 7440-44-0
|2 NLM Chemicals
|a Carbon
650 _ 7 |2 WoSType
|a J
653 2 0 |2 Author
|a C-11 tracer
653 2 0 |2 Author
|a Phloem leakage
653 2 0 |2 Author
|a Phloem transit times
653 2 0 |2 Author
|a Sucrose transporter
653 2 0 |2 Author
|a Transposon mutagenesis
700 1 _ |0 P:(DE-HGF)0
|a Thorpe, M. R.
|b 1
700 1 _ |0 P:(DE-HGF)0
|a Pritchard, J.
|b 2
700 1 _ |0 P:(DE-HGF)0
|a Christeller, J.I.
|b 3
700 1 _ |0 P:(DE-HGF)0
|a Williams, L.E.
|b 4
700 1 _ |0 P:(DE-Juel1)129390
|a Roeb, G.
|b 5
|u FZJ
700 1 _ |0 P:(DE-Juel1)129402
|a Schurr, U.
|b 6
|u FZJ
700 1 _ |0 P:(DE-Juel1)VDB23736
|a Minchin, P. E. H.
|b 7
|u FZJ
773 _ _ |0 PERI:(DE-600)1498605-x
|a 10.1016/j.plantsci.2011.12.018
|g Vol. 188-189, p. 97 - 101
|p 97 - 101
|q 188-189<97 - 101
|t Plant science
|v 188-189
|x 0168-9452
|y 2012
856 7 _ |u http://dx.doi.org/10.1016/j.plantsci.2011.12.018
856 4 _ |u https://juser.fz-juelich.de/record/19781/files/FZJ-19781.pdf
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|z Published final document.
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