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000019786 0247_ $$2DOI$$a10.3390/molecules17011055
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000019786 084__ $$2WoS$$aChemistry, Organic
000019786 1001_ $$0P:(DE-Juel1)VDB104414$$aKleusch, C.$$b0$$uFZJ
000019786 245__ $$aFluorescent lipids: functional parts of fusogenic liposomes and tools for cell membrane labeling and visualization
000019786 260__ $$aBasel$$bMDPI$$c2012
000019786 300__ $$a1055 - 1073
000019786 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000019786 440_0 $$024782$$aMolecules$$v17$$y1
000019786 500__ $$aWe thank Gerhild van Echten-Deckert for helpful discussions and Simon Ludwanowski, who produced many of the fusogenic liposomes used in this study. Our work was fully funded by the Helmholtz Association.
000019786 520__ $$aIn this paper a rapid and highly efficient method for controlled incorporation of fluorescent lipids into living mammalian cells is introduced. Here, the fluorescent molecules have two consecutive functions: First, they trigger rapid membrane fusion between cellular plasma membranes and the lipid bilayers of their carrier particles, so called fusogenic liposomes, and second, after insertion into cellular membranes these molecules enable fluorescence imaging of cell membranes and membrane traffic processes. We tested the fluorescent derivatives of the following essential membrane lipids for membrane fusion: Ceramide, sphingomyelin, phosphocholine, phosphatidylinositol-bisphosphate, ganglioside, cholesterol, and cholesteryl ester. Our results show that all probed lipids could more efficiently be incorporated into the plasma membrane of living cells than by using other methods. Moreover, labeling occurred in a gentle manner under classical cell culture conditions reducing cellular stress responses. Staining procedures were monitored by fluorescence microscopy and it was observed that sphingolipids and cholesterol containing free hydroxyl groups exhibit a decreased distribution velocity as well as a longer persistence in the plasma membrane compared to lipids without hydroxyl groups like phospholipids or other artificial lipid analogs. After membrane staining, the fluorescent molecules were sorted into membranes of cell organelles according to their chemical properties and biological functions without any influence of the delivery system.
000019786 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
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000019786 65320 $$2Author$$afusogenic liposomes
000019786 65320 $$2Author$$acellular membrane staining
000019786 65320 $$2Author$$afluorescent lipids
000019786 65320 $$2Author$$aDiR
000019786 65320 $$2Author$$aBodipy FL-sphingomyelin
000019786 65320 $$2Author$$afluorescence microscopy
000019786 650_2 $$2MeSH$$aAnimals
000019786 650_2 $$2MeSH$$aCHO Cells
000019786 650_2 $$2MeSH$$aCell Membrane: chemistry
000019786 650_2 $$2MeSH$$aCell Survival: drug effects
000019786 650_2 $$2MeSH$$aCricetinae
000019786 650_2 $$2MeSH$$aFibroblasts: chemistry
000019786 650_2 $$2MeSH$$aFluorescent Dyes: chemistry
000019786 650_2 $$2MeSH$$aIntracellular Membranes: chemistry
000019786 650_2 $$2MeSH$$aLiposomes: chemistry
000019786 650_2 $$2MeSH$$aMembrane Fusion: drug effects
000019786 650_2 $$2MeSH$$aMembrane Lipids: chemistry
000019786 650_2 $$2MeSH$$aMembrane Lipids: pharmacology
000019786 650_2 $$2MeSH$$aOrganelles: chemistry
000019786 650_2 $$2MeSH$$aRats
000019786 650_2 $$2MeSH$$aRats, Wistar
000019786 650_2 $$2MeSH$$aStaining and Labeling: methods
000019786 650_7 $$00$$2NLM Chemicals$$aFluorescent Dyes
000019786 650_7 $$00$$2NLM Chemicals$$aLiposomes
000019786 650_7 $$00$$2NLM Chemicals$$aMembrane Lipids
000019786 650_7 $$2WoSType$$aJ
000019786 7001_ $$0P:(DE-Juel1)VDB29396$$aHersch, N.$$b1$$uFZJ
000019786 7001_ $$0P:(DE-Juel1)VDB27696$$aHoffmann, B.$$b2$$uFZJ
000019786 7001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b3$$uFZJ
000019786 7001_ $$0P:(DE-Juel1)128805$$aCsiszar, A.$$b4$$uFZJ
000019786 773__ $$0PERI:(DE-600)2008644-1$$a10.3390/molecules17011055$$gVol. 17, p. 1055 - 1073$$p1055 - 1073$$q17<1055 - 1073$$tMolecules$$v17$$x1420-3049$$y2012
000019786 8567_ $$uhttp://dx.doi.org/10.3390/molecules17011055
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