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| 001 | 19786 | ||
| 005 | 20200423203146.0 | ||
| 024 | 7 | _ | |a pmid:22267193 |2 pmid |
| 024 | 7 | _ | |a 10.3390/molecules17011055 |2 DOI |
| 024 | 7 | _ | |a WOS:000299535700072 |2 WOS |
| 024 | 7 | _ | |a 1420-3049 |2 ISSN |
| 024 | 7 | _ | |a 2128/11532 |2 Handle |
| 024 | 7 | _ | |a altmetric:21807547 |2 altmetric |
| 037 | _ | _ | |a PreJuSER-19786 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 540 |
| 084 | _ | _ | |2 WoS |a Chemistry, Organic |
| 100 | 1 | _ | |a Kleusch, C. |b 0 |u FZJ |0 P:(DE-Juel1)VDB104414 |
| 245 | _ | _ | |a Fluorescent lipids: functional parts of fusogenic liposomes and tools for cell membrane labeling and visualization |
| 260 | _ | _ | |c 2012 |a Basel |b MDPI |
| 300 | _ | _ | |a 1055 - 1073 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Molecules |0 24782 |y 1 |v 17 |
| 500 | _ | _ | |a We thank Gerhild van Echten-Deckert for helpful discussions and Simon Ludwanowski, who produced many of the fusogenic liposomes used in this study. Our work was fully funded by the Helmholtz Association. |
| 520 | _ | _ | |a In this paper a rapid and highly efficient method for controlled incorporation of fluorescent lipids into living mammalian cells is introduced. Here, the fluorescent molecules have two consecutive functions: First, they trigger rapid membrane fusion between cellular plasma membranes and the lipid bilayers of their carrier particles, so called fusogenic liposomes, and second, after insertion into cellular membranes these molecules enable fluorescence imaging of cell membranes and membrane traffic processes. We tested the fluorescent derivatives of the following essential membrane lipids for membrane fusion: Ceramide, sphingomyelin, phosphocholine, phosphatidylinositol-bisphosphate, ganglioside, cholesterol, and cholesteryl ester. Our results show that all probed lipids could more efficiently be incorporated into the plasma membrane of living cells than by using other methods. Moreover, labeling occurred in a gentle manner under classical cell culture conditions reducing cellular stress responses. Staining procedures were monitored by fluorescence microscopy and it was observed that sphingolipids and cholesterol containing free hydroxyl groups exhibit a decreased distribution velocity as well as a longer persistence in the plasma membrane compared to lipids without hydroxyl groups like phospholipids or other artificial lipid analogs. After membrane staining, the fluorescent molecules were sorted into membranes of cell organelles according to their chemical properties and biological functions without any influence of the delivery system. |
| 536 | _ | _ | |a BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung |c P45 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK505 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Animals |
| 650 | _ | 2 | |2 MeSH |a CHO Cells |
| 650 | _ | 2 | |2 MeSH |a Cell Membrane: chemistry |
| 650 | _ | 2 | |2 MeSH |a Cell Survival: drug effects |
| 650 | _ | 2 | |2 MeSH |a Cricetinae |
| 650 | _ | 2 | |2 MeSH |a Fibroblasts: chemistry |
| 650 | _ | 2 | |2 MeSH |a Fluorescent Dyes: chemistry |
| 650 | _ | 2 | |2 MeSH |a Intracellular Membranes: chemistry |
| 650 | _ | 2 | |2 MeSH |a Liposomes: chemistry |
| 650 | _ | 2 | |2 MeSH |a Membrane Fusion: drug effects |
| 650 | _ | 2 | |2 MeSH |a Membrane Lipids: chemistry |
| 650 | _ | 2 | |2 MeSH |a Membrane Lipids: pharmacology |
| 650 | _ | 2 | |2 MeSH |a Organelles: chemistry |
| 650 | _ | 2 | |2 MeSH |a Rats |
| 650 | _ | 2 | |2 MeSH |a Rats, Wistar |
| 650 | _ | 2 | |2 MeSH |a Staining and Labeling: methods |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Fluorescent Dyes |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Liposomes |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Membrane Lipids |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a fusogenic liposomes |
| 653 | 2 | 0 | |2 Author |a cellular membrane staining |
| 653 | 2 | 0 | |2 Author |a fluorescent lipids |
| 653 | 2 | 0 | |2 Author |a DiR |
| 653 | 2 | 0 | |2 Author |a Bodipy FL-sphingomyelin |
| 653 | 2 | 0 | |2 Author |a fluorescence microscopy |
| 700 | 1 | _ | |a Hersch, N. |b 1 |u FZJ |0 P:(DE-Juel1)VDB29396 |
| 700 | 1 | _ | |a Hoffmann, B. |b 2 |u FZJ |0 P:(DE-Juel1)VDB27696 |
| 700 | 1 | _ | |a Merkel, R. |b 3 |u FZJ |0 P:(DE-Juel1)128833 |
| 700 | 1 | _ | |a Csiszar, A. |b 4 |u FZJ |0 P:(DE-Juel1)128805 |
| 773 | _ | _ | |0 PERI:(DE-600)2008644-1 |a 10.3390/molecules17011055 |g Vol. 17, p. 1055 - 1073 |p 1055 - 1073 |q 17<1055 - 1073 |t Molecules |v 17 |x 1420-3049 |y 2012 |
| 856 | 7 | _ | |u http://dx.doi.org/10.3390/molecules17011055 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/19786/files/molecules-17-01055.pdf |y OpenAccess |
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| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/19786/files/molecules-17-01055.pdf?subformat=pdfa |x pdfa |y OpenAccess |
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| 914 | 1 | _ | |y 2012 |
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