TY  - JOUR
AU  - Abagnale, Giulio
AU  - Steger, Michael
AU  - Nguyen, Vu Hoa
AU  - Hersch, Nils
AU  - Sechi, Antonio
AU  - Joussen, Sylvia
AU  - Denecke, Bernd
AU  - Merkel, Rudolf
AU  - Hoffmann, Bernd
AU  - Dreser, Alice
AU  - Schnakenberg, Uwe
AU  - Gillner, Arnold
AU  - Wagner, Wolfgang
TI  - Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages
JO  - Biomaterials
VL  - 61
SN  - 0142-9612
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2015-03281
SP  - 316 - 326
PY  - 2015
AB  - Surface topography impacts on cell growth and differentiation, but it is not trivial to generate defined surface structures and to assess the relevance of specific topographic parameters. In this study, we have systematically compared in vitro differentiation of mesenchymal stem cells (MSCs) on a variety of groove/ridge structures. Micro- and nano-patterns were generated in polyimide using reactive ion etching or multi beam laser interference, respectively. These structures affected cell spreading and orientation of human MSCs, which was also reflected in focal adhesions morphology and size. Time-lapse demonstrated directed migration parallel to the nano-patterns. Overall, surface patterns clearly enhanced differentiation of MSCs towards specific lineages: 15 μm ridges increased adipogenic differentiation whereas 2 μm ridges enhanced osteogenic differentiation. Notably, nano-patterns with a periodicity of 650 nm increased differentiation towards both osteogenic and adipogenic lineages. However, in absence of differentiation media surface structures did neither induce differentiation, nor lineage-specific gene expression changes. Furthermore, nanostructures did not affect the YAP/TAZ complex, which is activated by substrate stiffness. Our results provide further insight into how structuring of tailored biomaterials and implant interfaces – e.g. by multi beam laser interference in sub-micrometer scale – do not induce differentiation of MSCs per se, but support their directed differentiation.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000357229900030
C6  - pmid:26026844
DO  - DOI:10.1016/j.biomaterials.2015.05.030
UR  - https://juser.fz-juelich.de/record/200940
ER  -