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@ARTICLE{Abagnale:200940,
      author       = {Abagnale, Giulio and Steger, Michael and Nguyen, Vu Hoa and
                      Hersch, Nils and Sechi, Antonio and Joussen, Sylvia and
                      Denecke, Bernd and Merkel, Rudolf and Hoffmann, Bernd and
                      Dreser, Alice and Schnakenberg, Uwe and Gillner, Arnold and
                      Wagner, Wolfgang},
      title        = {{S}urface topography enhances differentiation of
                      mesenchymal stem cells towards osteogenic and adipogenic
                      lineages},
      journal      = {Biomaterials},
      volume       = {61},
      issn         = {0142-9612},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2015-03281},
      pages        = {316 - 326},
      year         = {2015},
      abstract     = {Surface topography impacts on cell growth and
                      differentiation, but it is not trivial to generate defined
                      surface structures and to assess the relevance of specific
                      topographic parameters. In this study, we have
                      systematically compared in vitro differentiation of
                      mesenchymal stem cells (MSCs) on a variety of groove/ridge
                      structures. Micro- and nano-patterns were generated in
                      polyimide using reactive ion etching or multi beam laser
                      interference, respectively. These structures affected cell
                      spreading and orientation of human MSCs, which was also
                      reflected in focal adhesions morphology and size. Time-lapse
                      demonstrated directed migration parallel to the
                      nano-patterns. Overall, surface patterns clearly enhanced
                      differentiation of MSCs towards specific lineages: 15 μm
                      ridges increased adipogenic differentiation whereas 2 μm
                      ridges enhanced osteogenic differentiation. Notably,
                      nano-patterns with a periodicity of 650 nm increased
                      differentiation towards both osteogenic and adipogenic
                      lineages. However, in absence of differentiation media
                      surface structures did neither induce differentiation, nor
                      lineage-specific gene expression changes. Furthermore,
                      nanostructures did not affect the YAP/TAZ complex, which is
                      activated by substrate stiffness. Our results provide
                      further insight into how structuring of tailored
                      biomaterials and implant interfaces – e.g. by multi beam
                      laser interference in sub-micrometer scale – do not induce
                      differentiation of MSCs per se, but support their directed
                      differentiation.},
      cin          = {ICS-7},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000357229900030},
      pubmed       = {pmid:26026844},
      doi          = {10.1016/j.biomaterials.2015.05.030},
      url          = {https://juser.fz-juelich.de/record/200940},
}