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@ARTICLE{Ortore:201077,
      author       = {Ortore, Maria Grazia and Mariani, Paolo and Carsughi,
                      Flavio and Cinelli, Stefania and Onori, Giuseppe and
                      Teixeira, José and Spinozzi, Francesco},
      title        = {{P}referential solvation of lysozyme in water/ethanol
                      mixtures},
      journal      = {The journal of chemical physics},
      volume       = {135},
      number       = {24},
      issn         = {0021-9606},
      address      = {Melville, NY},
      publisher    = {American Institute of Physics},
      reportid     = {FZJ-2015-03387},
      pages        = {245103},
      year         = {2011},
      abstract     = {We provide a quantitative description of the solvation
                      properties of lysozyme in water/ethanol mixtures, which has
                      been obtained by a simultaneous analysis of small-angle
                      neutron scattering and differential scanning calorimetry
                      experiments. All data sets were analyzed by an original
                      method, which integrates the exchange equilibrium model
                      between water and ethanol molecules at the protein surface
                      and activity coefficients data of water/ethanol binary
                      mixtures. As a result, the preferential binding of ethanol
                      molecules at the protein surface was obtained for both
                      native and thermal unfolded protein states. Excess solvation
                      numbers reveal a critical point at ethanolmolar fraction
                      ≈0.06, corresponding to the triggering of the hydrophobic
                      clustering of alcohol molecules detected in water/ethanol
                      binary mixtures.},
      cin          = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
                      (München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1 /
                      JCNS-2},
      ddc          = {540},
      cid          = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
                      I:(DE-Juel1)JCNS-1-20110106 / I:(DE-Juel1)JCNS-2-20110106},
      pnm          = {54G - JCNS (POF2-54G24)},
      pid          = {G:(DE-HGF)POF2-54G24},
      experiment   = {EXP:(DE-MLZ)External-20140101},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000298640500054},
      doi          = {10.1063/1.3670419},
      url          = {https://juser.fz-juelich.de/record/201077},
}