%0 Journal Article
%A Sandal, Massimo
%A Paltrinieri, Daniele
%A Carloni, Paolo
%A Musiani, Francesco
%A Giorgetti, Alejandro
%T Structure/Function Relationships of Phospholipases C Beta
%J Current protein & peptide science
%V 14
%N 8
%@ 1389-2037
%C Hilversum
%I Bentham Science Publ.
%M FZJ-2015-03608
%P 650 - 657
%D 2013
%X Phospholipases C beta (PLC-βs) are essential components of the signal transduction of metazoans. They catalyze the production of the second messengers inositol-1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) from the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PIP2). These enzymes are activated by G-protein-coupled receptors (GPCRs) through the interaction with the alpha subunit of heterotrimeric G-proteins belonging to the Gq family (Gαq), the Gβγ subunits released by the inhibitory G-protein (Gi) and Ca2+ ions. Here we review current structural insights on these important proteins, with a particular focus on the most structurally characterized isoform (PLC-β3) and the activation mechanism operated by Gαq. We propose, following the lead of recent studies, that a tight combination of experiments and molecular simulations are instrumental in further enlightening the structure/function understanding of PLC-βs. - See more at: http://www.eurekaselect.com/116059/article#sthash.cojH1BB1.dpuf
%F PUB:(DE-HGF)16 ; PUB:(DE-HGF)36
%9 Journal ArticleReview
%$ pmid:24384033
%U <Go to ISI:>//WOS:000329125100002
%R 10.2174/13892037113146660085
%U https://juser.fz-juelich.de/record/201303