TY  - JOUR
AU  - Musiani, Francesco
AU  - Ippoliti, Emiliano
AU  - Micheletti, Cristian
AU  - Carloni, Paolo
AU  - Ciurli, Stefano
TI  - Conformational Fluctuations of UreG, an Intrinsically Disordered Enzyme
JO  - Biochemistry
VL  - 52
IS  - 17
SN  - 1520-4995
CY  - Columbus, Ohio
PB  - American Chemical Society
M1  - FZJ-2015-03615
SP  - 2949 - 2954
PY  - 2013
AB  - UreG proteins are small GTP binding (G) proteins that catalyze the hydrolysis of GTP necessary for the maturation of urease, a virulence factor in bacterial pathogenesis. UreG proteins are the first documented cases of intrinsically disordered enzymes. The comprehension of the dynamics of folding−unfolding events occurring in this protein could shed light on the enzymatic mechanism of UreG. Here, we used the recently developed replica exchange with solute tempering (REST2) computational methodology to explore the conformational space of UreG from Helicobacter pylori (HpUreG) and to identify its structural fluctuations. The same simulation and analysis protocol has been applied to HypB from Methanocaldococcus jannaschii (MjHypB), which is closely related to UreG in both sequence and function, even though it is not intrinsically disordered. A comparison of the two systems reveals that both HpUreG and MjHypB feature a substantial rigidity of the protein regions involved in catalysis, justifying its residual catalytic activity. On the other hand, HpUreG tends to unfold more than MjHypB in portions involved in protein−protein interactions with metallochaperones necessary for the formation of multiprotein complexes known to be involved in urease activation.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000318333100010
DO  - DOI:10.1021/bi4001744
UR  - https://juser.fz-juelich.de/record/201310
ER  -