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000201330 1001_ $$0P:(DE-HGF)0$$aD’Urzo, Annalisa$$b0
000201330 245__ $$aMolecular Basis for Structural Heterogeneity of an Intrinsically Disordered Protein Bound to a Partner by Combined ESI-IM-MS and Modeling
000201330 260__ $$aNew York [u.a.]$$bSpringer$$c2015
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000201330 520__ $$aIntrinsically disordered proteins (IDPs) form biologically active complexes that can retain a high degree of conformational disorder, escaping structural characterization by conventional approaches. An example is offered by the complex between the intrinsically disordered NTAIL domain and the phosphoprotein X domain (PXD) from measles virus (MeV). Here, distinct conformers of the complex are detected by electrospray ionization-mass spectrometry (ESI-MS) and ion mobility (IM) techniques yielding estimates for the solvent-accessible surface area (SASA) in solution and the average collision cross-section (CCS) in the gas phase. Computational modeling of the complex in solution, based on experimental constraints, provides atomic-resolution structural models featuring different levels of compactness. The resulting models indicate high structural heterogeneity. The intermolecular interactions are predominantly hydrophobic, not only in the ordered core of the complex, but also in the dynamic, disordered regions. Electrostatic interactions become involved in the more compact states. This system represents an illustrative example of a hydrophobic complex that could be directly detected in the gas phase by native mass spectrometry. This work represents the first attempt to modeling the entire NTAIL domain bound to PXD at atomic resolution.
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000201330 7001_ $$0P:(DE-HGF)0$$aKonijnenberg, Albert$$b1
000201330 7001_ $$0P:(DE-Juel1)145921$$aRossetti, Giulia$$b2$$ufzj
000201330 7001_ $$0P:(DE-HGF)0$$aHabchi, Johnny$$b3
000201330 7001_ $$0P:(DE-Juel1)166112$$aLi, Jinyu$$b4$$ufzj
000201330 7001_ $$0P:(DE-Juel1)145614$$aCarloni, Paolo$$b5$$ufzj
000201330 7001_ $$0P:(DE-HGF)0$$aSobott, Frank$$b6
000201330 7001_ $$0P:(DE-HGF)0$$aLonghi, Sonia$$b7$$eCorresponding Author
000201330 7001_ $$0P:(DE-HGF)0$$aGrandori, Rita$$b8$$eCorresponding Author
000201330 773__ $$0PERI:(DE-600)2019911-9$$a10.1007/s13361-014-1048-z$$gVol. 26, no. 3, p. 472 - 481$$n3$$p472 - 481$$tJournal of the American Society for Mass Spectrometry$$v26$$x1879-1123$$y2015
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