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@ARTICLE{DUrzo:201330,
author = {D’Urzo, Annalisa and Konijnenberg, Albert and Rossetti,
Giulia and Habchi, Johnny and Li, Jinyu and Carloni, Paolo
and Sobott, Frank and Longhi, Sonia and Grandori, Rita},
title = {{M}olecular {B}asis for {S}tructural {H}eterogeneity of an
{I}ntrinsically {D}isordered {P}rotein {B}ound to a
{P}artner by {C}ombined {ESI}-{IM}-{MS} and {M}odeling},
journal = {Journal of the American Society for Mass Spectrometry},
volume = {26},
number = {3},
issn = {1879-1123},
address = {New York [u.a.]},
publisher = {Springer},
reportid = {FZJ-2015-03627},
pages = {472 - 481},
year = {2015},
abstract = {Intrinsically disordered proteins (IDPs) form biologically
active complexes that can retain a high degree of
conformational disorder, escaping structural
characterization by conventional approaches. An example is
offered by the complex between the intrinsically disordered
NTAIL domain and the phosphoprotein X domain (PXD) from
measles virus (MeV). Here, distinct conformers of the
complex are detected by electrospray ionization-mass
spectrometry (ESI-MS) and ion mobility (IM) techniques
yielding estimates for the solvent-accessible surface area
(SASA) in solution and the average collision cross-section
(CCS) in the gas phase. Computational modeling of the
complex in solution, based on experimental constraints,
provides atomic-resolution structural models featuring
different levels of compactness. The resulting models
indicate high structural heterogeneity. The intermolecular
interactions are predominantly hydrophobic, not only in the
ordered core of the complex, but also in the dynamic,
disordered regions. Electrostatic interactions become
involved in the more compact states. This system represents
an illustrative example of a hydrophobic complex that could
be directly detected in the gas phase by native mass
spectrometry. This work represents the first attempt to
modeling the entire NTAIL domain bound to PXD at atomic
resolution.},
cin = {INM-9 / IAS-5 / GRS / JSC},
ddc = {530},
cid = {I:(DE-Juel1)INM-9-20140121 / I:(DE-Juel1)IAS-5-20120330 /
I:(DE-Juel1)GRS-20100316 / I:(DE-Juel1)JSC-20090406},
pnm = {574 - Theory, modelling and simulation (POF3-574) / 511 -
Computational Science and Mathematical Methods (POF3-511)},
pid = {G:(DE-HGF)POF3-574 / G:(DE-HGF)POF3-511},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000350106100011},
pubmed = {pmid:25510932},
doi = {10.1007/s13361-014-1048-z},
url = {https://juser.fz-juelich.de/record/201330},
}