TY  - JOUR
AU  - Biarnés, Xevi
AU  - Marchiori, Alessandro
AU  - Giorgetti, Alejandro
AU  - Lanzara, Carmela
AU  - Gasparini, Paolo
AU  - Carloni, Paolo
AU  - Born, Stephan
AU  - Brockhoff, Anne
AU  - Behrens, Maik
AU  - Meyerhof, Wolfgang
TI  - Insights into the Binding of Phenyltiocarbamide (PTC) Agonist to Its Target Human TAS2R38 Bitter Receptor
JO  - PLoS one
VL  - 5
IS  - 8
SN  - 1932-6203
CY  - Lawrence, Kan.
PB  - PLoS
M1  - FZJ-2015-03638
SP  - e12394 -
PY  - 2010
AB  - Humans' bitter taste perception is mediated by the hTAS2R subfamily of the G protein-coupled membrane receptors (GPCRs). Structural information on these receptors is currently limited. Here we identify residues involved in the binding of phenylthiocarbamide (PTC) and in receptor activation in one of the most widely studied hTAS2Rs (hTAS2R38) by means of structural bioinformatics and molecular docking. The predictions are validated by site-directed mutagenesis experiments that involve specific residues located in the putative binding site and trans-membrane (TM) helices 6 and 7 putatively involved in receptor activation. Based on our measurements, we suggest that (i) residue N103 participates actively in PTC binding, in line with previous computational studies. (ii) W99, M100 and S259 contribute to define the size and shape of the binding cavity. (iii) W99 and M100, along with F255 and V296, play a key role for receptor activation, providing insights on bitter taste receptor activation not emerging from the previously reported computational models.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000281234700019
C6  - 20811630
DO  - DOI:10.1371/journal.pone.0012394
UR  - https://juser.fz-juelich.de/record/201341
ER  -