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@ARTICLE{Axer:20154,
author = {Axer, M. and Amunts, K. and Grässel, D. and Palm, C. and
Dammers, J. and Axer, H. and Pietrzyk, U. and Zilles, K.},
title = {{A} novel approach to the human connectome: ultra-high
resolution mapping of fiber tracts in the brain},
journal = {NeuroImage},
volume = {54},
issn = {1053-8119},
address = {Orlando, Fla.},
publisher = {Academic Press},
reportid = {PreJuSER-20154},
pages = {1091 - 1101},
year = {2011},
note = {We would like to thank M. Cremer, F. Kocaer and H.
Mohlberg, Research Centre Julich, Germany, and U. Blohm and
U. Opfermann, University Dusseldorf, Germany, for excellent
technical assistance and preparation of the histological
sections. Our work was partly supported by the Initiative
and Network Fund of the Helmholtz Association within the
Helmholtz Alliance on Systems Biology ("Human Brain
Model").},
abstract = {Signal transmission between different brain regions
requires connecting fiber tracts, the structural basis of
the human connectome. In contrast to animal brains, where a
multitude of tract tracing methods can be used, magnetic
resonance (MR)-based diffusion imaging is presently the only
promising approach to study fiber tracts between specific
human brain regions. However, this procedure has various
inherent restrictions caused by its relatively low spatial
resolution. Here, we introduce 3D-polarized light imaging
(3D-PLI) to map the three-dimensional course of fiber tracts
in the human brain with a resolution at a submillimeter
scale based on a voxel size of 100 μm isotropic or less.
3D-PLI demonstrates nerve fibers by utilizing their
intrinsic birefringence of myelin sheaths surrounding axons.
This optical method enables the demonstration of 3D fiber
orientations in serial microtome sections of entire human
brains. Examples for the feasibility of this novel approach
are given here. 3D-PLI enables the study of brain regions of
intense fiber crossing in unprecedented detail, and provides
an independent evaluation of fiber tracts derived from
diffusion imaging data.},
keywords = {Birefringence / Brain: ultrastructure / Brain Mapping:
methods / Humans / Image Processing, Computer-Assisted:
methods / Imaging, Three-Dimensional: methods / Nerve
Fibers: ultrastructure / Neural Pathways: anatomy $\&$
histology / J (WoSType)},
cin = {INM-4 / INM-1 / INM-2},
ddc = {610},
cid = {I:(DE-Juel1)INM-4-20090406 / I:(DE-Juel1)INM-1-20090406 /
I:(DE-Juel1)INM-2-20090406},
pnm = {Neurowissenschaften (FUEK255) / 333 - Pathophysiological
Mechanisms of Neurological and Psychiatric Diseases
(POF2-333)},
pid = {G:(DE-Juel1)FUEK255 / G:(DE-HGF)POF2-333},
shelfmark = {Neurosciences / Neuroimaging / Radiology, Nuclear Medicine
$\&$ Medical Imaging},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:20832489},
UT = {WOS:000285486000034},
doi = {10.1016/j.neuroimage.2010.08.075},
url = {https://juser.fz-juelich.de/record/20154},
}