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@ARTICLE{Baudoin:201603,
      author       = {Baudoin, Jean-Pierre and Jinschek, Joerg R. and Boothroyd,
                      Chris B. and Dunin-Borkowski, Rafal E. and de Jonge, Niels},
      title        = {{C}hromatic {A}berration-{C}orrected {T}ilt {S}eries
                      {T}ransmission {E}lectron {M}icroscopy of {N}anoparticles in
                      a {W}hole {M}ount {M}acrophage {C}ell},
      journal      = {Microscopy and microanalysis},
      volume       = {19},
      number       = {04},
      issn         = {1435-8115},
      address      = {New York, NY},
      publisher    = {Cambridge University Press},
      reportid     = {FZJ-2015-03897},
      pages        = {814 - 820},
      year         = {2013},
      abstract     = {Transmission electron microscopy (TEM) in combination with
                      electron tomography is widely used to obtain nanometer scale
                      three-dimensional (3D) structural information about
                      biological samples. However, studies of whole eukaryotic
                      cells are limited in resolution and/or contrast on account
                      of the effect of chromatic aberration of the TEM objective
                      lens on electrons that have been scattered inelastically in
                      the specimen. As a result, 3D information is usually
                      obtained from sections and not from whole cells. Here, we
                      use chromatic aberration-corrected TEM to record
                      bright-field TEM images of nanoparticles in a whole mount
                      macrophage cell. Tilt series of images are used to generate
                      electron tomograms, which are analyzed to assess the spatial
                      resolution that can be achieved for different vertical
                      positions in the specimen. The uptake of gold nanoparticles
                      coated with low-density lipoprotein (LDL) is studied. The
                      LDL is found to assemble in clusters. The clusters contain
                      nanoparticles taken up on different days, which are joined
                      without mixing their nanoparticle cargo.},
      cin          = {PGI-5},
      ddc          = {570},
      cid          = {I:(DE-Juel1)PGI-5-20110106},
      pnm          = {42G - Peter Grünberg-Centre (PG-C) (POF2-42G41)},
      pid          = {G:(DE-HGF)POF2-42G41},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000321764700006},
      doi          = {10.1017/S1431927613001475},
      url          = {https://juser.fz-juelich.de/record/201603},
}