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@ARTICLE{Schrader:20194,
author = {Schrader, T.E. and Cordes, T. and Schreier, W.J. and
Koller, F.O. and Dong, S.-L. and Moroder, L. and Zinth, W.},
title = {{F}olding and {U}nfolding of {L}ight {T}riggered
beta-{H}airpin {M}odel {P}eptides},
journal = {The journal of physical chemistry / B},
volume = {115},
issn = {1520-6106},
address = {Washington, DC},
publisher = {Soc.},
reportid = {PreJuSER-20194},
pages = {5219 - 5226},
year = {2011},
note = {The authors thank Alexander Milbradt, Christian Renner, and
Markus Loweneck for synthesis of the molecules and NMR
analysis, and Ulrike Kusebauch for helpful discussions.
Financial support from the Deutsche Forschungsgemeinschaft
(SFB 533 Projects A8, B9, and SFB 749, project A5) is highly
acknowledged. This work was also supported through the
DFG-Cluster of Excellence Munich-Centre for Advanced
Photonics. The authors thank Shaul Mukamel for many helpful
discussions on light triggered peptides.},
abstract = {Ultrafast spectroscopy in the visible and mid-infrared is
used to study the reaction dynamics of two light-triggered
model peptides containing an azobenzene derivative as a
switching element. One model peptide, the AzoTrpZip2, forms
a β-hairpin structure in the cis form of the chromophore.
This peptide is compared to the core structure consisting of
the chromophore and the two flanking amino acid residues,
used as a minimal model. This combination of experiments
performed in different spectral ranges on peptides of
different sizes allows for improved insight into light
triggered reaction dynamics. The kinetics observed for the
core structure are directly connected to the switching
process of the chromophore and are finished on the 10 ps
time scale. The trans-to-cis reaction of AzoTrpZip2, leading
to the formation of the β-hairpin structure involves
ultrafast processes on the 100 ps time scale, which are
directly related to the relaxation of the strain between the
isomerized molecular switch and the two peptide strands.
IR-signatures characteristic for changes in interstrand
interactions are absent on the <1 ns time scale. Thus
folding into the β-hairpin structure occurs on a much
longer time scale. In the cis-to-trans unfolding reaction,
all IR signatures related to changes in interstrand
interactions occur within 1 ns, in a time range where
visible spectroscopy reveals the final decay of the
intramolecular strain. Apparently unfolding of AzoTrpZip2 is
to a large extent a fast, driven process.},
keywords = {Amino Acid Sequence / Azo Compounds: chemistry / Inverted
Repeat Sequences / Peptides: chemistry / Protein Folding /
Protein Structure, Secondary / Protein Unfolding /
Spectrophotometry, Infrared / Azo Compounds (NLM Chemicals)
/ Peptides (NLM Chemicals) / azobenzene (NLM Chemicals) / J
(WoSType)},
cin = {ICS-1 / JCNS (München) ; Jülich Centre for Neutron
Science JCNS (München) ; JCNS-FRM-II / JCNS-1},
ddc = {530},
cid = {I:(DE-Juel1)ICS-1-20110106 /
I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {BioSoft: Makromolekulare Systeme und biologische
Informationsverarbeitung / Großgeräte für die Forschung
mit Photonen, Neutronen und Ionen (PNI)},
pid = {G:(DE-Juel1)FUEK505 / G:(DE-Juel1)FUEK415},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
shelfmark = {Chemistry, Physical},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:21175125},
UT = {WOS:000290127100018},
doi = {10.1021/jp107683d},
url = {https://juser.fz-juelich.de/record/20194},
}