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@ARTICLE{Sgobba:202096,
author = {Sgobba, Miriam and Olubiyi, Olujide and Ke, Song and
Haider, Shozeb},
title = {{M}olecular dynamics of {HIV}1-integrase in complex with
93del - a structural perspective on the mechanism of
inhibition.},
journal = {Caryologia},
volume = {29},
number = {5},
issn = {1538-0254},
address = {Abingdon, Oxon},
publisher = {Taylor $\&$ Francis63883},
reportid = {FZJ-2015-04385},
pages = {863 - 877},
year = {2012},
abstract = {HIV1 integrase is an important target for the antiviral
therapy. Guanine-rich quadruplex, such as 93del, have been
shown to be potent inhibitors of this enzyme and thus
representing a new class of antiviral agents. Although X-ray
and NMR structures of HIV1 integrase and 93del have been
reported, there is no structural information of the complex
and the mechanism of inhibition still remains unexplored. A
number of computational methods including automated
protein-DNA docking and molecular dynamics simulation in
explicit solvent were used to model the binding of 93del to
HIV1 integrase. Analysis of the dynamic behaviour of the
complex using principal components analysis and elastic
network modelling techniques allow us to understand how the
binding of 93del aptamer and its interactions with key
residues affect the intrinsic motions of the catalytic loops
by stabilising them in catalytically inactive conformations.
Such insights into the structural mechanism of inhibition
can aid in improving the design of anti-HIV aptamers.},
keywords = {Aptamers, Nucleotide (NLM Chemicals) / HIV Integrase
Inhibitors (NLM Chemicals) / p31 integrase protein, Human
immunodeficiency virus 1 (NLM Chemicals) / HIV Integrase
(NLM Chemicals)},
cin = {ICS-6},
ddc = {570},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {452 - Structural Biology (POF2-452)},
pid = {G:(DE-HGF)POF2-452},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:22292948},
UT = {WOS:000300074600002},
doi = {10.1080/07391102.2012.10507418},
url = {https://juser.fz-juelich.de/record/202096},
}