%0 Journal Article
%A Ishchenko, A.
%A Round, E.
%A Borshchevskiy, V.
%A Grudinin, S.
%A Gushchin, I.
%A Klare, J. P.
%A Balandin, T.
%A Remeeva, A.
%A Engelhard, M.
%A Büldt, G.
%A Gordeliy, V.
%T Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer
%J Journal of photochemistry and photobiology / B
%V 123
%@ 1011-1344
%C New York, NY [u.a.]
%I Elsevier
%M FZJ-2015-04422
%P 55 - 58
%D 2013
%X The complex of sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII) mediates negative phototaxis in halobacteria Natronomonas pharaonis. Upon light activation NpSRII triggers, by means of NpHtrII, a signal transduction chain homologous to the two component system in eubacterial chemotaxis. Here we report on the crystal structure of the ground state of the mutant NpSRII-D75N/NpHtrII complex in the space group I212121. Mutations of this aspartic acid in light-driven proton pumps dramatically modify or/and inhibit protein functions. However, in vivo studies show that the similar D75N mutation retains functionality of the NpSRII/NpHtrII complex. The structure provides the molecular basis for the explanation of the unexpected observation that the wild and the mutant complexes display identical physiological response on light excitation.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:23619282
%U <Go to ISI:>//WOS:000319544100007
%R 10.1016/j.jphotobiol.2013.03.008
%U https://juser.fz-juelich.de/record/202133