TY  - JOUR
AU  - Ishchenko, A.
AU  - Round, E.
AU  - Borshchevskiy, V.
AU  - Grudinin, S.
AU  - Gushchin, I.
AU  - Klare, J. P.
AU  - Balandin, T.
AU  - Remeeva, A.
AU  - Engelhard, M.
AU  - Büldt, G.
AU  - Gordeliy, V.
TI  - Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer
JO  - Journal of photochemistry and photobiology / B
VL  - 123
SN  - 1011-1344
CY  - New York, NY [u.a.]
PB  - Elsevier
M1  - FZJ-2015-04422
SP  - 55 - 58
PY  - 2013
AB  - The complex of sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII) mediates negative phototaxis in halobacteria Natronomonas pharaonis. Upon light activation NpSRII triggers, by means of NpHtrII, a signal transduction chain homologous to the two component system in eubacterial chemotaxis. Here we report on the crystal structure of the ground state of the mutant NpSRII-D75N/NpHtrII complex in the space group I212121. Mutations of this aspartic acid in light-driven proton pumps dramatically modify or/and inhibit protein functions. However, in vivo studies show that the similar D75N mutation retains functionality of the NpSRII/NpHtrII complex. The structure provides the molecular basis for the explanation of the unexpected observation that the wild and the mutant complexes display identical physiological response on light excitation.
LB  - PUB:(DE-HGF)16
C6  - pmid:23619282
UR  - <Go to ISI:>//WOS:000319544100007
DO  - DOI:10.1016/j.jphotobiol.2013.03.008
UR  - https://juser.fz-juelich.de/record/202133
ER  -