%0 Journal Article
%A Gushchin, Ivan
%A Shevchenko, Vitaly
%A Polovinkin, V.
%A Kovalev, Kirill
%A Alekseev, Alexey
%A Round, E.
%A Borshchevskiy, Valentin
%A Balandin, Taras
%A Popov, A.
%A Gensch, Thomas
%A Fahlke, Christoph
%A Bamann, C.
%A Willbold, Dieter
%A Büldt, Georg
%A Bamberg, E.
%A Gordeliy, Valentin
%T Crystal structure of a light-driven sodium pump
%J Nature structural & molecular biology
%V 22
%N 5
%@ 1545-9993
%C New York, NY
%I Nature America
%M FZJ-2015-04449
%P 390-395
%D 2015
%X Recently, the first known light-driven sodium pumps, from the microbial rhodopsin family, were discovered. We have solved the structure of one of them, Krokinobacter eikastus ​rhodopsin 2 (​KR2), in the monomeric blue state and in two pentameric red states, at resolutions of 1.45 Å and 2.2 and 2.8 Å, respectively. The structures reveal the ion-translocation pathway and show that the sodium ion is bound outside the protein at the oligomerization interface, that the ion-release cavity is capped by a unique N-terminal α-helix and that the ion-uptake cavity is unexpectedly large and open to the surface. Obstruction of the cavity with the mutation G263F imparts ​KR2 with the ability to pump potassium. These results pave the way for the understanding and rational design of cation pumps with new specific properties valuable for optogenetics.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000354094700012
%$ pmid:25849142
%R 10.1038/nsmb.3002
%U https://juser.fz-juelich.de/record/202160