TY  - JOUR
AU  - Gushchin, Ivan
AU  - Shevchenko, Vitaly
AU  - Polovinkin, V.
AU  - Kovalev, Kirill
AU  - Alekseev, Alexey
AU  - Round, E.
AU  - Borshchevskiy, Valentin
AU  - Balandin, Taras
AU  - Popov, A.
AU  - Gensch, Thomas
AU  - Fahlke, Christoph
AU  - Bamann, C.
AU  - Willbold, Dieter
AU  - Büldt, Georg
AU  - Bamberg, E.
AU  - Gordeliy, Valentin
TI  - Crystal structure of a light-driven sodium pump
JO  - Nature structural & molecular biology
VL  - 22
IS  - 5
SN  - 1545-9993
CY  - New York, NY
PB  - Nature America
M1  - FZJ-2015-04449
SP  - 390-395
PY  - 2015
AB  - Recently, the first known light-driven sodium pumps, from the microbial rhodopsin family, were discovered. We have solved the structure of one of them, Krokinobacter eikastus ​rhodopsin 2 (​KR2), in the monomeric blue state and in two pentameric red states, at resolutions of 1.45 Å and 2.2 and 2.8 Å, respectively. The structures reveal the ion-translocation pathway and show that the sodium ion is bound outside the protein at the oligomerization interface, that the ion-release cavity is capped by a unique N-terminal α-helix and that the ion-uptake cavity is unexpectedly large and open to the surface. Obstruction of the cavity with the mutation G263F imparts ​KR2 with the ability to pump potassium. These results pave the way for the understanding and rational design of cation pumps with new specific properties valuable for optogenetics.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000354094700012
C6  - pmid:25849142
DO  - DOI:10.1038/nsmb.3002
UR  - https://juser.fz-juelich.de/record/202160
ER  -