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@ARTICLE{Poojari:202185,
author = {Poojari, Chetan},
title = {{A}ggregation of {A}myloids at {B}iomembranes and its
{I}mplications in {A}lzheimers's {D}isease and {T}ype {II}
{D}iabetes},
journal = {Biophysical journal},
volume = {104},
number = {2},
issn = {0006-3495},
address = {New York, NY},
publisher = {Rockefeller Univ. Press},
reportid = {FZJ-2015-04474},
pages = {592a -},
year = {2013},
abstract = {The aggregation of the amyloid-β peptide (Aβ) into
neurotoxic oligomers on the neuronal membrane surface and
its insertion into the membrane is considered to be a
crucial event in the development of Alzheimer's disease
(AD). However, the mechanism of insertion, pore formation
and membrane disruption still needs to be uncovered. We used
atomistic molecular dynamics (MD) simulations to investigate
the behavior of Aβ in zwitterionic and anionic lipid
bilayers. We studied the effect of Aβ secondary structure,
oligomerization and mutation on its transmembrane stability
and membrane maintenance. Our main finding is that β
sheet-oligomerization is required for Aβto be stable in the
membrane and to induce membrane permeabilization.Aggregation
of human islet amyloid polypeptide (hIAPP) at beta-cell
membranes is associated with the onset of type II diabetes.
It is proposed that hIAPP aggregates induce cytotoxicity to
the pancreatic islets of langerhans cells by membrane
disruption. Chiral surface-specific vibrational sum
frequency generation (SFG) spectroscopy in conjunction with
ab initio simulations revealed a tilted orientation of hIAPP
β sheet-aggregates at lipid/aqueous interfaces. We used
this orientation for the starting structure of a hIAPP
trimer inserted into a lipid bilayer and followed its
effects on membrane maintenance using MD simulations. We
observe β barrel-formation, which allows massive water and
even ion flow across the membrane.},
cin = {ICS-6},
ddc = {570},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {452 - Structural Biology (POF2-452)},
pid = {G:(DE-HGF)POF2-452},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000316074305536},
doi = {10.1016/j.bpj.2012.11.3291},
url = {https://juser.fz-juelich.de/record/202185},
}