TY  - JOUR
AU  - Tang, Yunqing
AU  - Dai, Luru
AU  - Zhang, Xiaoming
AU  - Li, Junbai
AU  - Hendriks, Johnny
AU  - Fan, Xiaoming
AU  - Gruteser, Nadine
AU  - Meisenberg, Annika
AU  - Baumann, A.
AU  - Katranidis, Alexandros
AU  - Gensch, Thomas
TI  - SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy
JO  - Scientific reports
VL  - 5
SN  - 2045-2322
CY  - London
PB  - Nature Publishing Group
M1  - FZJ-2015-04565
SP  - 11073 -
PY  - 2015
AB  - Single molecule localization based super-resolution fluorescence microscopy offers significantly higher spatial resolution than predicted by Abbe’s resolution limit for far field optical microscopy. Such super-resolution images are reconstructed from wide-field or total internal reflection single molecule fluorescence recordings. Discrimination between emission of single fluorescent molecules and background noise fluctuations remains a great challenge in current data analysis. Here we present a real-time, and robust single molecule identification and localization algorithm, SNSMIL (Shot Noise based Single Molecule Identification and Localization). This algorithm is based on the intrinsic nature of noise, i.e., its Poisson or shot noise characteristics and a new identification criterion, QSNSMIL, is defined. SNSMIL improves the identification accuracy of single fluorescent molecules in experimental or simulated datasets with high and inhomogeneous background. The implementation of SNSMIL relies on a graphics processing unit (GPU), making real-time analysis feasible as shown for real experimental and simulated datasets.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000356584200001
C6  - pmid:26098742
DO  - DOI:10.1038/srep11073
UR  - https://juser.fz-juelich.de/record/202294
ER  -