TY  - JOUR
AU  - Kopka, Benita
AU  - Diener, Martin
AU  - Wirtz, Astrid
AU  - Pohl, Martina
AU  - Jaeger, Karl-Erich
AU  - Krauss, Ulrich
TI  - Purification and simultaneous immobilization of Arabidopsis thaliana hydroxynitrile Lyase using a Family 2 carbohydrate-binding module
JO  - Biotechnology journal
VL  - 10
IS  - 5
SN  - 1860-6768
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2015-04682
SP  - 811 - 819
PY  - 2015
AB  - Tedious, time- and labor-intensive protein purification and immobilization procedures still represent a major bottleneck limiting the widespread application of enzymes in synthetic chemistry and industry. We here exemplify a simple strategy for the direct site-specific immobilization of proteins from crude cell extracts by fusion of a family 2 carbohydrate-binding module (CBM) derived from the exoglucanase/xylanase Cex from Cellulomonas fimi to a target enzyme. By employing a tripartite fusion protein consisting of the CBM, a flavin-based fluorescent protein (FbFP), and the Arabidopsis thaliana hydroxynitrile lyase (AtHNL), binding to cellulosic carrier materials can easily be monitored via FbFP fluorescence. Adsorption properties (kinetics and quantities) were studied for commercially available Avicel PH-101 and regenerated amorphous cellulose (RAC) derived from Avicel. The resulting immobilizates showed similar activities as the wild-type enzyme but displayed increased stability in the weakly acidic pH range. Finally, Avicel, RAC and cellulose acetate (CA) preparations were used for the synthesis of (R)-mandelonitrile in micro-aqueous methyl tert-butyl ether (MTBE) demonstrating the applicability and stability of the immobilizates for biotransformations in both aqueous and organic reaction systems.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000354199700016
C6  - pmid:25755120
DO  - DOI:10.1002/biot.201400786
UR  - https://juser.fz-juelich.de/record/202468
ER  -