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@ARTICLE{Kopka:202468,
      author       = {Kopka, Benita and Diener, Martin and Wirtz, Astrid and
                      Pohl, Martina and Jaeger, Karl-Erich and Krauss, Ulrich},
      title        = {{P}urification and simultaneous immobilization of
                      {A}rabidopsis thaliana hydroxynitrile {L}yase using a
                      {F}amily 2 carbohydrate-binding module},
      journal      = {Biotechnology journal},
      volume       = {10},
      number       = {5},
      issn         = {1860-6768},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2015-04682},
      pages        = {811 - 819},
      year         = {2015},
      abstract     = {Tedious, time- and labor-intensive protein purification and
                      immobilization procedures still represent a major bottleneck
                      limiting the widespread application of enzymes in synthetic
                      chemistry and industry. We here exemplify a simple strategy
                      for the direct site-specific immobilization of proteins from
                      crude cell extracts by fusion of a family 2
                      carbohydrate-binding module (CBM) derived from the
                      exoglucanase/xylanase Cex from Cellulomonas fimi to a target
                      enzyme. By employing a tripartite fusion protein consisting
                      of the CBM, a flavin-based fluorescent protein (FbFP), and
                      the Arabidopsis thaliana hydroxynitrile lyase (AtHNL),
                      binding to cellulosic carrier materials can easily be
                      monitored via FbFP fluorescence. Adsorption properties
                      (kinetics and quantities) were studied for commercially
                      available Avicel PH-101 and regenerated amorphous cellulose
                      (RAC) derived from Avicel. The resulting immobilizates
                      showed similar activities as the wild-type enzyme but
                      displayed increased stability in the weakly acidic pH range.
                      Finally, Avicel, RAC and cellulose acetate (CA) preparations
                      were used for the synthesis of (R)-mandelonitrile in
                      micro-aqueous methyl tert-butyl ether (MTBE) demonstrating
                      the applicability and stability of the immobilizates for
                      biotransformations in both aqueous and organic reaction
                      systems.},
      cin          = {IBG-1 / IMET},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118 / I:(DE-Juel1)IMET-20090612},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000354199700016},
      pubmed       = {pmid:25755120},
      doi          = {10.1002/biot.201400786},
      url          = {https://juser.fz-juelich.de/record/202468},
}