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@ARTICLE{Spiegel:202944,
      author       = {Spiegel, Michaela and Duraisamy, Amudha Kumari and
                      Schröder, Gunnar},
      title        = {{I}mproving the visualization of cryo-{EM} density
                      reconstructions},
      journal      = {Journal of structural biology},
      volume       = {191},
      number       = {2},
      issn         = {1047-8477},
      address      = {San Diego, Calif.},
      publisher    = {Elsevier},
      reportid     = {FZJ-2015-05067},
      pages        = {207 - 213},
      year         = {2015},
      abstract     = {Cryo-electron microscopy yields 3D density maps of
                      macromolecules from single-particle images, tomograms, or 2D
                      crystals. An optimal visualization of the density map is
                      important for its proper interpretation. We have developed a
                      method to improve the visualization of density maps by using
                      general statistical information about proteins for the
                      sharpening process. In particular, the packing density of
                      atoms is highly similar between different proteins, which
                      allows for building a pseudo-atomic model to approximate the
                      true mass distribution. From this model the radial structure
                      factor and density value histogram are estimated and applied
                      as constraints to the 3D reconstruction in reciprocal- and
                      real-space, respectively. Interestingly, similar
                      improvements are obtained when using the correct radial
                      structure factor and density value histogram from a crystal
                      structure. Thus, the estimated pseudo-atomic model yields a
                      sufficiently accurate mass distribution to optimally sharpen
                      a density map.},
      cin          = {ICS-6},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000359096700014},
      doi          = {10.1016/j.jsb.2015.06.007},
      url          = {https://juser.fz-juelich.de/record/202944},
}