%0 Journal Article
%A Rösch, Sarah
%A Johnen, Sandra
%A Mazinani, Babac
%A Müller, Frank
%A Pfarrer, Christiane
%A Walter, Peter
%T The effects of iodoacetic acid on the mouse retina
%J Graefe's archive for clinical and experimental ophthalmology
%V 253
%N 1
%@ 1435-702X
%C Berlin
%I Springer
%M FZJ-2015-05121
%P 25 - 35
%D 2015
%X Background:To characterize the effects of intravitreal injections of iodoacetic acid (IAA) in comparison to its systemic application as a measure to induce unilateral photoreceptor degeneration.Methods:Seven-week-old C57BL/6 J mice received either intravitreal injections of IAA or systemic treatment (intraperitoneal vs intravenous) and were observed in the following 5 weeks using ERG, OCT, and histology.Results:Systemic treatment with IAA induced high toxic effects and a high mortality in contrast to the intravitreal injection. Intraperitoneal application had no effect on the retina. Intravenous application of 2 × 30 mg/kg BW IAA (time between injections 3.5 h) resulted in an extinction of the ERG and a thinning of the retina, in particular of the outer nuclear layer (ONL) indicating photoreceptor degeneration. Animals receiving intravitreal injections developed cataracts already at low concentrations (up to 100 % at 0.25 mg/kg BW). Higher intravitreal IAA doses led to extinguished ERGs. In histology, a thinning of the entire retina was observed that was most prominent in the inner part of the retina.Conclusions:In contrast to intraperitoneal administration, intravenous application of IAA led to a selective photoreceptor degeneration. After intravitreal injection, dense cataracts were already observed at concentrations lower than those needed to induce changes in the ERG. ERG results must be interpreted carefully. A thinning of all retinal layers rather than a specific outer retinal degeneration was observed upon intravitreal injection. IAA is not a useful model to induce outer retinal degeneration in mice.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000347553200004
%$ pmid:24827634
%R 10.1007/s00417-014-2652-0
%U https://juser.fz-juelich.de/record/203086