TY  - JOUR
AU  - Rösch, Sarah
AU  - Johnen, Sandra
AU  - Mazinani, Babac
AU  - Müller, Frank
AU  - Pfarrer, Christiane
AU  - Walter, Peter
TI  - The effects of iodoacetic acid on the mouse retina
JO  - Graefe's archive for clinical and experimental ophthalmology
VL  - 253
IS  - 1
SN  - 1435-702X
CY  - Berlin
PB  - Springer
M1  - FZJ-2015-05121
SP  - 25 - 35
PY  - 2015
AB  - Background:To characterize the effects of intravitreal injections of iodoacetic acid (IAA) in comparison to its systemic application as a measure to induce unilateral photoreceptor degeneration.Methods:Seven-week-old C57BL/6 J mice received either intravitreal injections of IAA or systemic treatment (intraperitoneal vs intravenous) and were observed in the following 5 weeks using ERG, OCT, and histology.Results:Systemic treatment with IAA induced high toxic effects and a high mortality in contrast to the intravitreal injection. Intraperitoneal application had no effect on the retina. Intravenous application of 2 × 30 mg/kg BW IAA (time between injections 3.5 h) resulted in an extinction of the ERG and a thinning of the retina, in particular of the outer nuclear layer (ONL) indicating photoreceptor degeneration. Animals receiving intravitreal injections developed cataracts already at low concentrations (up to 100 % at 0.25 mg/kg BW). Higher intravitreal IAA doses led to extinguished ERGs. In histology, a thinning of the entire retina was observed that was most prominent in the inner part of the retina.Conclusions:In contrast to intraperitoneal administration, intravenous application of IAA led to a selective photoreceptor degeneration. After intravitreal injection, dense cataracts were already observed at concentrations lower than those needed to induce changes in the ERG. ERG results must be interpreted carefully. A thinning of all retinal layers rather than a specific outer retinal degeneration was observed upon intravitreal injection. IAA is not a useful model to induce outer retinal degeneration in mice.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000347553200004
C6  - pmid:24827634
DO  - DOI:10.1007/s00417-014-2652-0
UR  - https://juser.fz-juelich.de/record/203086
ER  -