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000020326 084__ $$2WoS$$aCell Biology
000020326 1001_ $$0P:(DE-Juel1)VDB19870$$aWeigel, S.$$b0$$uFZJ
000020326 245__ $$aLocust primary neuronal culture for the study of synaptic transmission
000020326 260__ $$aDordrecht [u.a.]$$bSpringer Science + Business Media B.V.$$c2012
000020326 300__ $$a405 - 419
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000020326 440_0 $$026568$$aJournal of Molecular Histology$$v43$$y4
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000020326 500__ $$aThis work was in part supported by the EC project CICADA of the IST Programme FET Key Action Life Like Perception funded in FP 5. We are very grateful to M. Knierim-Grenzebach for her help and advice with neuronal cell culture. We thank A. Reska for her initial work in the early phase of this project and M. Gebhardt and K. Gobbels for critical comments on the manuscript.
000020326 520__ $$aWe have designed a cell culture system for thoracic neurons of adult Locusta migratoria that enables the establishment of functional synapses in vitro. Patch-clamp recordings revealed three different neuron classes. About half of the neurons (47%) had unexcitable somata with outward and no inward conductance. The other half generated either single (37%) or multiple action potentials (18%) and differed mainly in lower outward conductance. Selectively stained motor neurons were analyzed to demonstrate varied physiological properties due to culture conditions. Using paired patch clamp recordings we demonstrate directly synaptic transmission in morphologically connected neurons in vitro. Presynaptic stimulation resulted in postsynaptic potentials in 42 pairs of neurons tested, independent of the type of neuron. According to pharmacological experiments most of these synapses were either glutamatergic or GABAergic. In addition to these chemical synapses, electrical synapses were found. With the demonstration of synapse formation in cell culture of adult locust neurons, this study provides the basis for the future analysis of more defined insect neuronal circuits in culture.
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000020326 65320 $$2Author$$aLocust
000020326 65320 $$2Author$$aCell culture
000020326 65320 $$2Author$$aChemical synapse
000020326 65320 $$2Author$$aElectrical synapse
000020326 65320 $$2Author$$aPatch-clamp
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000020326 7001_ $$0P:(DE-Juel1)VDB19869$$aSchulte, P.$$b1$$uFZJ
000020326 7001_ $$0P:(DE-Juel1)VDB71557$$aMeffert, S.$$b2$$uFZJ
000020326 7001_ $$0P:(DE-HGF)0$$aBräunig, P.$$b3
000020326 7001_ $$0P:(DE-Juel1)128713$$aOffenhäusser, A.$$b4$$uFZJ
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