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@ARTICLE{Weigel:20326,
      author       = {Weigel, S. and Schulte, P. and Meffert, S. and Bräunig, P.
                      and Offenhäusser, A.},
      title        = {{L}ocust primary neuronal culture for the study of synaptic
                      transmission},
      journal      = {Journal of molecular histology},
      volume       = {43},
      issn         = {1567-2379},
      address      = {Dordrecht [u.a.]},
      publisher    = {Springer Science + Business Media B.V.},
      reportid     = {PreJuSER-20326},
      pages        = {405 - 419},
      year         = {2012},
      note         = {This work was in part supported by the EC project CICADA of
                      the IST Programme FET Key Action Life Like Perception funded
                      in FP 5. We are very grateful to M. Knierim-Grenzebach for
                      her help and advice with neuronal cell culture. We thank A.
                      Reska for her initial work in the early phase of this
                      project and M. Gebhardt and K. Gobbels for critical comments
                      on the manuscript.},
      abstract     = {We have designed a cell culture system for thoracic neurons
                      of adult Locusta migratoria that enables the establishment
                      of functional synapses in vitro. Patch-clamp recordings
                      revealed three different neuron classes. About half of the
                      neurons $(47\%)$ had unexcitable somata with outward and no
                      inward conductance. The other half generated either single
                      $(37\%)$ or multiple action potentials $(18\%)$ and differed
                      mainly in lower outward conductance. Selectively stained
                      motor neurons were analyzed to demonstrate varied
                      physiological properties due to culture conditions. Using
                      paired patch clamp recordings we demonstrate directly
                      synaptic transmission in morphologically connected neurons
                      in vitro. Presynaptic stimulation resulted in postsynaptic
                      potentials in 42 pairs of neurons tested, independent of the
                      type of neuron. According to pharmacological experiments
                      most of these synapses were either glutamatergic or
                      GABAergic. In addition to these chemical synapses,
                      electrical synapses were found. With the demonstration of
                      synapse formation in cell culture of adult locust neurons,
                      this study provides the basis for the future analysis of
                      more defined insect neuronal circuits in culture.},
      keywords     = {J (WoSType)},
      cin          = {ICS-8 / JARA-FIT / PGI-8},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-8-20110106 / $I:(DE-82)080009_20140620$ /
                      I:(DE-Juel1)PGI-8-20110106},
      pnm          = {Grundlagen für zukünftige Informationstechnologien},
      pid          = {G:(DE-Juel1)FUEK412},
      shelfmark    = {Cell Biology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:22403023},
      UT           = {WOS:000308325900004},
      doi          = {10.1007/s10735-012-9395-1},
      url          = {https://juser.fz-juelich.de/record/20326},
}