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001     20326
005     20240619091011.0
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|a 10.1007/s10735-012-9395-1
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037 _ _ |a PreJuSER-20326
041 _ _ |a eng
082 _ _ |a 540
084 _ _ |2 WoS
|a Cell Biology
100 1 _ |0 P:(DE-Juel1)VDB19870
|a Weigel, S.
|b 0
|u FZJ
245 _ _ |a Locust primary neuronal culture for the study of synaptic transmission
260 _ _ |a Dordrecht [u.a.]
|b Springer Science + Business Media B.V.
|c 2012
300 _ _ |a 405 - 419
336 7 _ |a Journal Article
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440 _ 0 |0 26568
|a Journal of Molecular Histology
|v 43
|y 4
500 _ _ |3 POF3_Assignment on 2016-02-29
500 _ _ |a This work was in part supported by the EC project CICADA of the IST Programme FET Key Action Life Like Perception funded in FP 5. We are very grateful to M. Knierim-Grenzebach for her help and advice with neuronal cell culture. We thank A. Reska for her initial work in the early phase of this project and M. Gebhardt and K. Gobbels for critical comments on the manuscript.
520 _ _ |a We have designed a cell culture system for thoracic neurons of adult Locusta migratoria that enables the establishment of functional synapses in vitro. Patch-clamp recordings revealed three different neuron classes. About half of the neurons (47%) had unexcitable somata with outward and no inward conductance. The other half generated either single (37%) or multiple action potentials (18%) and differed mainly in lower outward conductance. Selectively stained motor neurons were analyzed to demonstrate varied physiological properties due to culture conditions. Using paired patch clamp recordings we demonstrate directly synaptic transmission in morphologically connected neurons in vitro. Presynaptic stimulation resulted in postsynaptic potentials in 42 pairs of neurons tested, independent of the type of neuron. According to pharmacological experiments most of these synapses were either glutamatergic or GABAergic. In addition to these chemical synapses, electrical synapses were found. With the demonstration of synapse formation in cell culture of adult locust neurons, this study provides the basis for the future analysis of more defined insect neuronal circuits in culture.
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653 2 0 |2 Author
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653 2 0 |2 Author
|a Cell culture
653 2 0 |2 Author
|a Chemical synapse
653 2 0 |2 Author
|a Electrical synapse
653 2 0 |2 Author
|a Patch-clamp
700 1 _ |0 P:(DE-Juel1)VDB19869
|a Schulte, P.
|b 1
|u FZJ
700 1 _ |0 P:(DE-Juel1)VDB71557
|a Meffert, S.
|b 2
|u FZJ
700 1 _ |0 P:(DE-HGF)0
|a Bräunig, P.
|b 3
700 1 _ |0 P:(DE-Juel1)128713
|a Offenhäusser, A.
|b 4
|u FZJ
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|a 10.1007/s10735-012-9395-1
|g Vol. 43, p. 405 - 419
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|t Journal of molecular histology
|v 43
|x 1567-2379
|y 2012
856 7 _ |u http://dx.doi.org/10.1007/s10735-012-9395-1
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