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@ARTICLE{BarbozaBarquero:203474,
      author       = {Barboza-Barquero, Luis and Nagel, Kerstin and Jansen,
                      Marcus and Klasen, Jonas R. and Kastenholz, Bernd and Braun,
                      Silvia and Bleise, Birgit and Brehm, Thorsten and Koornneef,
                      Maarten and Fiorani, Fabio},
      title        = {{P}henotype of {A}rabidopsis thaliana semi-dwarfs with deep
                      roots and high growth rates under water-limiting conditions
                      is independent of the {GA}5 loss-of-function alleles},
      journal      = {Annals of botany},
      volume       = {116},
      number       = {3},
      issn         = {0305-7364},
      address      = {Oxford},
      publisher    = {Oxford University Press},
      reportid     = {FZJ-2015-05404},
      pages        = {321-331},
      year         = {2015},
      abstract     = {Background and Aims The occurrence of Arabidopsis thaliana
                      semi-dwarf accessions carrying inactive alleles at the
                      gibberellin (GA) biosynthesis GA5 locus has raised the
                      question whether there are pleiotropic effects on other
                      traits at the root level, such as rooting depth. In
                      addition, it is unknown whether semi-dwarfism in arabidopsis
                      confers a growth advantage under water-limiting conditions
                      compared with wild-type plants. The aim of this research was
                      therefore to investigate whether semi-dwarfism has a
                      pleiotropic effect in the root system and also whether
                      semi-dwarfs might be more tolerant of water-limiting
                      conditions.Methods The root systems of different arabidopsis
                      semi-dwarfs and GA biosynthesis mutants were phenotyped in
                      vitro using the GROWSCREEN-ROOT image-based software.
                      Semi-dwarfs were phenotyped together with tall, near-related
                      accessions. In addition, root phenotypes were investigated
                      in soil-filled rhizotrons. Rosette growth trajectories were
                      analysed with the GROWSCREEN-FLUORO setup based on
                      non-invasive imaging.Key Results Mutations in the early
                      steps of the GA biosynthesis pathway led to a reduction in
                      shoot as well as root size. Depending on the genetic
                      background, mutations at the GA5 locus yielded phenotypes
                      characterized by decreased root length in comparison with
                      related wild-type ones. The semi-dwarf accession Pak-3
                      showed the deepest root system both in vitro and in soil
                      cultivation experiments; this comparatively deep root
                      system, however, was independent of the ga5 loss-of-function
                      allele, as shown by co-segregation analysis. When the
                      accessions were grown under water-limiting conditions,
                      semi-dwarf accessions with high growth rates were
                      identified.Conclusions The observed diversity in root system
                      growth and architecture occurs independently of semi-dwarf
                      phenotypes, and is probably linked to a genetic background
                      effect. The results show that there are no clear advantages
                      of semi-dwarfism at low water availability in arabidopsis.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000361392300002},
      pubmed       = {pmid:26162399},
      doi          = {10.1093/aob/mcv099},
      url          = {https://juser.fz-juelich.de/record/203474},
}