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000020489 0247_ $$2DOI$$a10.1002/elps.201100381
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000020489 084__ $$2WoS$$aBiochemical Research Methods
000020489 084__ $$2WoS$$aChemistry, Analytical
000020489 1001_ $$0P:(DE-Juel1)VDB94855$$aPosch, T.N.$$b0$$uFZJ
000020489 245__ $$aImplementation of a design of experiments to study the influence of the background electrolyte on separation and detection in non-aqueous capillary electrophoresis-mass spectrometry
000020489 260__ $$aWeinheim$$bWiley-Blackwell$$c2012
000020489 300__ $$a583 - 598
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000020489 440_0 $$01792$$aElectrophoresis$$v33$$x0173-0835$$y4
000020489 500__ $$3POF3_Assignment on 2016-02-29
000020489 500__ $$aThe help of Jorg Roscher is gratefully acknowledged. We thank the Helmholtz Initiative and Networking Fund for financial support.
000020489 520__ $$aNon-aqueous capillary electrophoresis (NACE) background electrolytes are most often composed of a mixture of methanol and acetonitrile (ACN) with soluble ammonium salts added as electrolyte. In this study on NACE-MS, we used a mixture of glacial acetic acid and ACN giving rise to an acidic background electrolyte (BGE) with a very low dielectric constant. Impressive changes in selectivity and resolution were observed for structurally closely related indole alkaloids including diastereomers upon addition of ammonium formate as electrolyte and upon variation of the solvent ratio. In order to obtain best separation and MS detection conditions and to reveal the influence of the parameters of the BGE on separation and detection and vice versa of the MS parameters on separation, an optimization strategy was employed using a design of experiments in a central composite design with response surface methodology. It was proven that at high electroosmotic flow conditions capillary electrophoretic separations and thus optimization can be realized without interference from the coupling to an MS system. Several significantly interacting parameters were revealed, which are not accessible with classical univariate optimization approaches. With this optimization, alkaloid mixtures from a plant extract of Mitragyna speciosa, containing a large number of diastereomeric compounds were successfully separated.
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000020489 65320 $$2Author$$aDesign of experiments
000020489 65320 $$2Author$$aHeteroconjugation
000020489 65320 $$2Author$$aIndole alkaloids
000020489 65320 $$2Author$$aIon-pairing
000020489 65320 $$2Author$$aNon-aqueous capillary electrophoresis
000020489 650_2 $$2MeSH$$aAcetic Acid: chemistry
000020489 650_2 $$2MeSH$$aAcetonitriles: chemistry
000020489 650_2 $$2MeSH$$aAnalysis of Variance
000020489 650_2 $$2MeSH$$aElectrolytes: chemistry
000020489 650_2 $$2MeSH$$aElectrophoresis, Capillary: methods
000020489 650_2 $$2MeSH$$aIndole Alkaloids: analysis
000020489 650_2 $$2MeSH$$aIndole Alkaloids: isolation & purification
000020489 650_2 $$2MeSH$$aMass Spectrometry: methods
000020489 650_2 $$2MeSH$$aMitragyna: chemistry
000020489 650_2 $$2MeSH$$aModels, Chemical
000020489 650_2 $$2MeSH$$aPlant Extracts: chemistry
000020489 650_2 $$2MeSH$$aResearch Design
000020489 650_7 $$00$$2NLM Chemicals$$aAcetonitriles
000020489 650_7 $$00$$2NLM Chemicals$$aElectrolytes
000020489 650_7 $$00$$2NLM Chemicals$$aIndole Alkaloids
000020489 650_7 $$00$$2NLM Chemicals$$aPlant Extracts
000020489 650_7 $$064-19-7$$2NLM Chemicals$$aAcetic Acid
000020489 650_7 $$075-05-8$$2NLM Chemicals$$aacetonitrile
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000020489 7001_ $$0P:(DE-HGF)0$$aMüller, A.$$b1
000020489 7001_ $$0P:(DE-HGF)0$$aSchulz, W.$$b2
000020489 7001_ $$0P:(DE-HGF)0$$aPütz, M.$$b3
000020489 7001_ $$0P:(DE-Juel1)VDB93008$$aHuhn, C.$$b4$$uFZJ
000020489 773__ $$0PERI:(DE-600)1475486-1$$a10.1002/elps.201100381$$gVol. 33, p. 583 - 598$$p583 - 598$$q33<583 - 598$$tElectrophoresis$$v33$$x0173-0835$$y2012
000020489 8567_ $$uhttp://dx.doi.org/10.1002/elps.201100381
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