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@ARTICLE{Posch:20489,
      author       = {Posch, T.N. and Müller, A. and Schulz, W. and Pütz, M.
                      and Huhn, C.},
      title        = {{I}mplementation of a design of experiments to study the
                      influence of the background electrolyte on separation and
                      detection in non-aqueous capillary electrophoresis-mass
                      spectrometry},
      journal      = {Electrophoresis},
      volume       = {33},
      issn         = {0173-0835},
      address      = {Weinheim},
      publisher    = {Wiley-Blackwell},
      reportid     = {PreJuSER-20489},
      pages        = {583 - 598},
      year         = {2012},
      note         = {The help of Jorg Roscher is gratefully acknowledged. We
                      thank the Helmholtz Initiative and Networking Fund for
                      financial support.},
      abstract     = {Non-aqueous capillary electrophoresis (NACE) background
                      electrolytes are most often composed of a mixture of
                      methanol and acetonitrile (ACN) with soluble ammonium salts
                      added as electrolyte. In this study on NACE-MS, we used a
                      mixture of glacial acetic acid and ACN giving rise to an
                      acidic background electrolyte (BGE) with a very low
                      dielectric constant. Impressive changes in selectivity and
                      resolution were observed for structurally closely related
                      indole alkaloids including diastereomers upon addition of
                      ammonium formate as electrolyte and upon variation of the
                      solvent ratio. In order to obtain best separation and MS
                      detection conditions and to reveal the influence of the
                      parameters of the BGE on separation and detection and vice
                      versa of the MS parameters on separation, an optimization
                      strategy was employed using a design of experiments in a
                      central composite design with response surface methodology.
                      It was proven that at high electroosmotic flow conditions
                      capillary electrophoretic separations and thus optimization
                      can be realized without interference from the coupling to an
                      MS system. Several significantly interacting parameters were
                      revealed, which are not accessible with classical univariate
                      optimization approaches. With this optimization, alkaloid
                      mixtures from a plant extract of Mitragyna speciosa,
                      containing a large number of diastereomeric compounds were
                      successfully separated.},
      keywords     = {Acetic Acid: chemistry / Acetonitriles: chemistry /
                      Analysis of Variance / Electrolytes: chemistry /
                      Electrophoresis, Capillary: methods / Indole Alkaloids:
                      analysis / Indole Alkaloids: isolation $\&$ purification /
                      Mass Spectrometry: methods / Mitragyna: chemistry / Models,
                      Chemical / Plant Extracts: chemistry / Research Design /
                      Acetonitriles (NLM Chemicals) / Electrolytes (NLM Chemicals)
                      / Indole Alkaloids (NLM Chemicals) / Plant Extracts (NLM
                      Chemicals) / Acetic Acid (NLM Chemicals) / acetonitrile (NLM
                      Chemicals) / J (WoSType)},
      cin          = {ZCH},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ZCH-20090406},
      pnm          = {Techniken, Innovation und Gesellschaft (TIG)},
      pid          = {G:(DE-Juel1)FUEK506},
      shelfmark    = {Biochemical Research Methods / Chemistry, Analytical},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:22451051},
      UT           = {WOS:000302010700007},
      doi          = {10.1002/elps.201100381},
      url          = {https://juser.fz-juelich.de/record/20489},
}