Home > Publications database > Implementation of a design of experiments to study the influence of the background electrolyte on separation and detection in non-aqueous capillary electrophoresis-mass spectrometry > print

001     20489
005     20180208220755.0
024 7 _ |2 pmid
|a pmid:22451051
024 7 _ |2 DOI
|a 10.1002/elps.201100381
024 7 _ |2 WOS
|a WOS:000302010700007
037 _ _ |a PreJuSER-20489
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biochemical Research Methods
084 _ _ |2 WoS
|a Chemistry, Analytical
100 1 _ |a Posch, T.N.
|b 0
|u FZJ
|0 P:(DE-Juel1)VDB94855
245 _ _ |a Implementation of a design of experiments to study the influence of the background electrolyte on separation and detection in non-aqueous capillary electrophoresis-mass spectrometry
260 _ _ |a Weinheim
|b Wiley-Blackwell
|c 2012
300 _ _ |a 583 - 598
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Electrophoresis
|x 0173-0835
|0 1792
|y 4
|v 33
500 _ _ |3 POF3_Assignment on 2016-02-29
500 _ _ |a The help of Jorg Roscher is gratefully acknowledged. We thank the Helmholtz Initiative and Networking Fund for financial support.
520 _ _ |a Non-aqueous capillary electrophoresis (NACE) background electrolytes are most often composed of a mixture of methanol and acetonitrile (ACN) with soluble ammonium salts added as electrolyte. In this study on NACE-MS, we used a mixture of glacial acetic acid and ACN giving rise to an acidic background electrolyte (BGE) with a very low dielectric constant. Impressive changes in selectivity and resolution were observed for structurally closely related indole alkaloids including diastereomers upon addition of ammonium formate as electrolyte and upon variation of the solvent ratio. In order to obtain best separation and MS detection conditions and to reveal the influence of the parameters of the BGE on separation and detection and vice versa of the MS parameters on separation, an optimization strategy was employed using a design of experiments in a central composite design with response surface methodology. It was proven that at high electroosmotic flow conditions capillary electrophoretic separations and thus optimization can be realized without interference from the coupling to an MS system. Several significantly interacting parameters were revealed, which are not accessible with classical univariate optimization approaches. With this optimization, alkaloid mixtures from a plant extract of Mitragyna speciosa, containing a large number of diastereomeric compounds were successfully separated.
536 _ _ |a Techniken, Innovation und Gesellschaft (TIG)
|c P47
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK506
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Acetic Acid: chemistry
650 _ 2 |2 MeSH
|a Acetonitriles: chemistry
650 _ 2 |2 MeSH
|a Analysis of Variance
650 _ 2 |2 MeSH
|a Electrolytes: chemistry
650 _ 2 |2 MeSH
|a Electrophoresis, Capillary: methods
650 _ 2 |2 MeSH
|a Indole Alkaloids: analysis
650 _ 2 |2 MeSH
|a Indole Alkaloids: isolation & purification
650 _ 2 |2 MeSH
|a Mass Spectrometry: methods
650 _ 2 |2 MeSH
|a Mitragyna: chemistry
650 _ 2 |2 MeSH
|a Models, Chemical
650 _ 2 |2 MeSH
|a Plant Extracts: chemistry
650 _ 2 |2 MeSH
|a Research Design
650 _ 7 |0 0
|2 NLM Chemicals
|a Acetonitriles
650 _ 7 |0 0
|2 NLM Chemicals
|a Electrolytes
650 _ 7 |0 0
|2 NLM Chemicals
|a Indole Alkaloids
650 _ 7 |0 0
|2 NLM Chemicals
|a Plant Extracts
650 _ 7 |0 64-19-7
|2 NLM Chemicals
|a Acetic Acid
650 _ 7 |0 75-05-8
|2 NLM Chemicals
|a acetonitrile
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a Design of experiments
653 2 0 |2 Author
|a Heteroconjugation
653 2 0 |2 Author
|a Indole alkaloids
653 2 0 |2 Author
|a Ion-pairing
653 2 0 |2 Author
|a Non-aqueous capillary electrophoresis
700 1 _ |a Müller, A.
|b 1
|0 P:(DE-HGF)0
700 1 _ |a Schulz, W.
|b 2
|0 P:(DE-HGF)0
700 1 _ |a Pütz, M.
|b 3
|0 P:(DE-HGF)0
700 1 _ |a Huhn, C.
|b 4
|u FZJ
|0 P:(DE-Juel1)VDB93008
773 _ _ |a 10.1002/elps.201100381
|g Vol. 33, p. 583 - 598
|p 583 - 598
|q 33<583 - 598
|0 PERI:(DE-600)1475486-1
|t Electrophoresis
|v 33
|y 2012
|x 0173-0835
856 7 _ |u http://dx.doi.org/10.1002/elps.201100381
909 C O |o oai:juser.fz-juelich.de:20489
|p VDB
913 1 _ |b Schlüsseltechnologien
|k P47
|l Technologien, Innovationen und Gesellschaft
|1 G:(DE-HGF)POF2-470
|0 G:(DE-Juel1)FUEK506
|2 G:(DE-HGF)POF2-100
|v Techniken, Innovation und Gesellschaft (TIG)
|x 0
|z vormals P26
913 2 _ |a DE-HGF
|b Forschungsbereich Energie
|l Technologie, Innovation und Gesellschaft
|1 G:(DE-HGF)POF3-150
|0 G:(DE-HGF)POF3-159H
|2 G:(DE-HGF)POF3-100
|v Addenda
|x 0
914 1 _ |y 2012
915 _ _ |a JCR/ISI refereed
|0 StatID:(DE-HGF)0010
|2 StatID
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
915 _ _ |a WoS
|0 StatID:(DE-HGF)0110
|2 StatID
|b Science Citation Index
915 _ _ |a WoS
|0 StatID:(DE-HGF)0111
|2 StatID
|b Science Citation Index Expanded
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Thomson Reuters Master Journal List
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0310
|2 StatID
|b NCBI Molecular Biology Database
915 _ _ |a Nationallizenz
|0 StatID:(DE-HGF)0420
|2 StatID
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
920 1 _ |k ZCH
|l Zentralabteilung für Chemische Analysen
|g ZCH
|0 I:(DE-Juel1)ZCH-20090406
|x 0
970 _ _ |a VDB:(DE-Juel1)136037
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a journal
980 _ _ |a I:(DE-Juel1)ZEA-3-20090406
980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)ZEA-3-20090406
981 _ _ |a I:(DE-Juel1)ZCH-20090406

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21