% IMPORTANT: The following is UTF-8 encoded. This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.
@ARTICLE{Kulig:20493,
author = {Kulig, J. and Simon, R.C. and Rose, C.A. and Husain, S.M.
and Häckh, M. and Lüdeke, S. and Zeitler, K. and Kroutil,
W. and Pohl, M. and Rother, D.},
title = {{S}tereoselective synthesis of bulky 1,2-diols with alcohol
dehydrogenases},
journal = {Catalysis Science $\&$ Technology},
volume = {2},
issn = {2044-4753},
reportid = {PreJuSER-20493},
pages = {1580 - 1589},
year = {2012},
note = {This work was financed by the Marie Curie Initial Training
Network "BIOTRAINS-a European biotechnology training network
for the support of chemical manufacturing'', grant agreement
no. 238531, and partially financed by the DFG (German
Research Foundation) in frame of the Research Group FOR
1296.},
abstract = {Although biotransformations implementing alcohol
dehydrogenases (ADHs) are widespread, enzymes which catalyse
the reduction and oxidation of sterically demanding
substrates, especially 2-hydroxy ketones, are still rare. To
fill this gap eight ADHs were investigated concerning their
potential to reduce bulky 2-hydroxy ketones. All of these
enzymes showed good activities along with excellent enantio(
ee > $99\%)$ and diastereoselectivities (de > $99\%).$ Due
to their differences in substrate preferences and
stereoselectivity a broad range of diastereomerically pure
1,2-diols is now accessible via biotransformation. Best
results were obtained using the alcohol dehydrogenase from
Ralstonia sp. (Cupriavidus sp.) (RADH), which showed a broad
substrate range, especially for sterically demanding
compounds. Araliphatic 2-hydroxy ketones, like
(R)-2-hydroxy-1-phenylpropan-1-one ((R)-2-HPP), were reduced
much faster than aliphatic or aromatic aldehydes (e. g.
benzaldehyde) under the applied conditions. Additionally
(R)-as well as (S)-2-hydroxy ketones were converted with
high diastereoselectivities (de > $99\%).$ RADH, which was
up to now only studied as a whole cell biocatalyst
overexpressed in E. coli, was purified and thoroughly
characterised concerning its catalytic properties.},
keywords = {J (WoSType)},
cin = {IBT-2},
cid = {I:(DE-Juel1)VDB56},
pnm = {Biotechnologie / BIOTRAINS - A EUROPEAN BIOTECHNOLOGY
TRAINING NETWORK FOR THE SUPPORT OF CHEMICAL MANUFACTURING
(238531)},
pid = {G:(DE-Juel1)FUEK410 / G:(EU-Grant)238531},
shelfmark = {Chemistry, Physical},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000306229800011},
doi = {10.1039/c2cy20120h},
url = {https://juser.fz-juelich.de/record/20493},
}