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@ARTICLE{Kulig:20493,
      author       = {Kulig, J. and Simon, R.C. and Rose, C.A. and Husain, S.M.
                      and Häckh, M. and Lüdeke, S. and Zeitler, K. and Kroutil,
                      W. and Pohl, M. and Rother, D.},
      title        = {{S}tereoselective synthesis of bulky 1,2-diols with alcohol
                      dehydrogenases},
      journal      = {Catalysis Science $\&$ Technology},
      volume       = {2},
      issn         = {2044-4753},
      reportid     = {PreJuSER-20493},
      pages        = {1580 - 1589},
      year         = {2012},
      note         = {This work was financed by the Marie Curie Initial Training
                      Network "BIOTRAINS-a European biotechnology training network
                      for the support of chemical manufacturing'', grant agreement
                      no. 238531, and partially financed by the DFG (German
                      Research Foundation) in frame of the Research Group FOR
                      1296.},
      abstract     = {Although biotransformations implementing alcohol
                      dehydrogenases (ADHs) are widespread, enzymes which catalyse
                      the reduction and oxidation of sterically demanding
                      substrates, especially 2-hydroxy ketones, are still rare. To
                      fill this gap eight ADHs were investigated concerning their
                      potential to reduce bulky 2-hydroxy ketones. All of these
                      enzymes showed good activities along with excellent enantio(
                      ee > $99\%)$ and diastereoselectivities (de > $99\%).$ Due
                      to their differences in substrate preferences and
                      stereoselectivity a broad range of diastereomerically pure
                      1,2-diols is now accessible via biotransformation. Best
                      results were obtained using the alcohol dehydrogenase from
                      Ralstonia sp. (Cupriavidus sp.) (RADH), which showed a broad
                      substrate range, especially for sterically demanding
                      compounds. Araliphatic 2-hydroxy ketones, like
                      (R)-2-hydroxy-1-phenylpropan-1-one ((R)-2-HPP), were reduced
                      much faster than aliphatic or aromatic aldehydes (e. g.
                      benzaldehyde) under the applied conditions. Additionally
                      (R)-as well as (S)-2-hydroxy ketones were converted with
                      high diastereoselectivities (de > $99\%).$ RADH, which was
                      up to now only studied as a whole cell biocatalyst
                      overexpressed in E. coli, was purified and thoroughly
                      characterised concerning its catalytic properties.},
      keywords     = {J (WoSType)},
      cin          = {IBT-2},
      cid          = {I:(DE-Juel1)VDB56},
      pnm          = {Biotechnologie / BIOTRAINS - A EUROPEAN BIOTECHNOLOGY
                      TRAINING NETWORK FOR THE SUPPORT OF CHEMICAL MANUFACTURING
                      (238531)},
      pid          = {G:(DE-Juel1)FUEK410 / G:(EU-Grant)238531},
      shelfmark    = {Chemistry, Physical},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000306229800011},
      doi          = {10.1039/c2cy20120h},
      url          = {https://juser.fz-juelich.de/record/20493},
}