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000205012 1001_ $$0P:(DE-HGF)0$$aCalligari, Paolo A.$$b0$$eCorresponding author
000205012 245__ $$aAdaptation of Extremophilic Proteins with Temperature and Pressure: Evidence from Initiation Factor 6
000205012 260__ $$aWashington, DC$$bSoc.$$c2015
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000205012 520__ $$aIn this work, we study dynamical properties of an extremophilic protein, Initiation Factor 6 (IF6), produced by the archeabacterium Methanocaldococcus jannascii, which thrives close to deep-sea hydrothermal vents where temperatures reach 80 °C and the pressure is up to 750 bar. Molecular dynamics simulations (MD) and quasi-elastic neutron scattering (QENS) measurements give new insights into the dynamical properties of this protein with respect to its eukaryotic and mesophilic homologue. Results obtained by MD are supported by QENS data and are interpreted within the framework of a fractional Brownian dynamics model for the characterization of protein relaxation dynamics. IF6 from M. jannaschii at high temperature and pressure shares similar flexibility with its eukaryotic homologue from S. cerevisieae under ambient conditions. This work shows for the first time, to our knowledge, that the very common pattern of corresponding states for thermophilic protein adaptation can be extended to thermo-barophilic proteins. A detailed analysis of dynamic properties and of local structural fluctuations reveals a complex pattern for “corresponding” structural flexibilities. In particular, in the case of IF6, the latter seems to be strongly related to the entropic contribution given by an additional, C-terminal, 20 amino-acid tail which is evolutionary conserved in all mesophilic IF6s.
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000205012 7001_ $$0P:(DE-Juel1)166168$$aCalandrini, Vania$$b1$$ufzj
000205012 7001_ $$0P:(DE-HGF)0$$aOllivier, Jacques$$b2
000205012 7001_ $$0P:(DE-HGF)0$$aArtero, Jean-Baptiste$$b3
000205012 7001_ $$0P:(DE-HGF)0$$aHärtlein, Michael$$b4
000205012 7001_ $$0P:(DE-HGF)0$$aJohnson, Mark$$b5$$eCorresponding author
000205012 7001_ $$0P:(DE-HGF)0$$aKneller, Gerald R.$$b6$$eCorresponding author
000205012 773__ $$0PERI:(DE-600)2006039-7$$a10.1021/acs.jpcb.5b02034$$gVol. 119, no. 25, p. 7860 - 7873$$n25$$p7860 - 7873$$tThe @journal of physical chemistry <Washington, DC> / B$$v119$$x1520-5207$$y2015
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