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@ARTICLE{Calligari:205012,
author = {Calligari, Paolo A. and Calandrini, Vania and Ollivier,
Jacques and Artero, Jean-Baptiste and Härtlein, Michael and
Johnson, Mark and Kneller, Gerald R.},
title = {{A}daptation of {E}xtremophilic {P}roteins with
{T}emperature and {P}ressure: {E}vidence from {I}nitiation
{F}actor 6},
journal = {The journal of physical chemistry / B},
volume = {119},
number = {25},
issn = {1520-5207},
address = {Washington, DC},
publisher = {Soc.},
reportid = {FZJ-2015-05517},
pages = {7860 - 7873},
year = {2015},
abstract = {In this work, we study dynamical properties of an
extremophilic protein, Initiation Factor 6 (IF6), produced
by the archeabacterium Methanocaldococcus jannascii, which
thrives close to deep-sea hydrothermal vents where
temperatures reach 80 °C and the pressure is up to 750 bar.
Molecular dynamics simulations (MD) and quasi-elastic
neutron scattering (QENS) measurements give new insights
into the dynamical properties of this protein with respect
to its eukaryotic and mesophilic homologue. Results obtained
by MD are supported by QENS data and are interpreted within
the framework of a fractional Brownian dynamics model for
the characterization of protein relaxation dynamics. IF6
from M. jannaschii at high temperature and pressure shares
similar flexibility with its eukaryotic homologue from S.
cerevisieae under ambient conditions. This work shows for
the first time, to our knowledge, that the very common
pattern of corresponding states for thermophilic protein
adaptation can be extended to thermo-barophilic proteins. A
detailed analysis of dynamic properties and of local
structural fluctuations reveals a complex pattern for
“corresponding” structural flexibilities. In particular,
in the case of IF6, the latter seems to be strongly related
to the entropic contribution given by an additional,
C-terminal, 20 amino-acid tail which is evolutionary
conserved in all mesophilic IF6s.},
cin = {GRS / IAS-5},
ddc = {530},
cid = {I:(DE-Juel1)GRS-20100316 / I:(DE-Juel1)IAS-5-20120330},
pnm = {899 - ohne Topic (POF3-899)},
pid = {G:(DE-HGF)POF3-899},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000357139800009},
pubmed = {pmid:25996652},
doi = {10.1021/acs.jpcb.5b02034},
url = {https://juser.fz-juelich.de/record/205012},
}