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@INPROCEEDINGS{Meier:205072,
author = {Meier, G. and Banachowicz, E. and Poznar, M. and Patkowski,
A. and Gapinski, J. and Kohlbrecher, J. and
Wilk-Kohlbrecher, A.},
title = {{S}tructure of the {I}ntrinsically {D}isordered {P}rotein
{O}tholith in {S}olution at {D}ifferent {P}rotein and {S}alt
{C}oncentrations},
reportid = {FZJ-2015-05548},
year = {2015},
abstract = {Two members of the intrinsically disordered proteins (IDP)
family (OMM-64 and STM) have been studied by means of small
angle neutron scattering (SANS), dynamic light scattering
(DLS) and fluorescence correlation spectroscopy (FCS). SANS
curves of the proteins in D2O buffers have been measured for
a range of Na+ and Ca2+ salts concentrations. The same
samples have been measured using DLS in order to estimate
their apparent hydrodynamic radii (Rh) and the scattering
intensity in the q = 0 limit. The size of the proteins as a
function of Na+ and Ca2+ concentrations had been measured in
nano-molar concentrations using FCS. Fits to the SANS data
suggest a coiled structure of the proteins in solution. For
the first time SANS curves of proteins embedded in calcium
carbonate crystals have been measured. Also a coiled
structure has been found (slope = -2). Exact values of the
radius of gyration (Rg) from SANS need measurements at
smaller q-values.},
month = {Aug},
date = {2015-08-30},
organization = {ECNS2015, Zaragoza (Spain), 30 Aug
2015 - 4 Sep 2015},
subtyp = {Other},
cin = {ICS-3},
cid = {I:(DE-Juel1)ICS-3-20110106},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)6},
url = {https://juser.fz-juelich.de/record/205072},
}