TY  - JOUR
AU  - Menzel, Miriam
AU  - Michielsen, Kristel
AU  - De Raedt, H.
AU  - Reckfort, Julia
AU  - Amunts, Katrin
AU  - Axer, Markus
TI  - A Jones matrix formalism for simulating three-dimensional polarized light imaging of brain tissue
JO  - Interface
VL  - 12
IS  - 111
SN  - 1742-5662
CY  - London
PB  - The Royal Society
M1  - FZJ-2015-05614
SP  - 20150734
PY  - 2015
AB  - The neuroimaging technique three-dimensional polarized light imaging (3D-PLI) provides a high-resolution reconstruction of nerve fibres in human post-mortem brains. The orientations of the fibres are derived from birefringence measurements of histological brain sections assuming that the nerve fibres—consisting of an axon and a surrounding myelin sheath—are uniaxial birefringent and that the measured optic axis is oriented in the direction of the nerve fibres (macroscopic model). Although experimental studies support this assumption, the molecular structure of the myelin sheath suggests that the birefringence of a nerve fibre can be described more precisely by multiple optic axes oriented radially around the fibre axis (microscopic model). In this paper, we compare the use of the macroscopic and the microscopic model for simulating 3D-PLI by means of the Jones matrix formalism. The simulations show that the macroscopic model ensures a reliable estimation of the fibre orientations as long as the polarimeter does not resolve structures smaller than the diameter of single fibres. In the case of fibre bundles, polarimeters with even higher resolutions can be used without losing reliability. When taking the myelin density into account, the derived fibre orientations are considerably improved.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000363487600027
C6  - pmid:26446561
DO  - DOI:10.1098/rsif.2015.0734
UR  - https://juser.fz-juelich.de/record/205172
ER  -