000020870 001__ 20870
000020870 005__ 20180208194459.0
000020870 0247_ $$2pmid$$apmid:22050934
000020870 0247_ $$2DOI$$a10.1016/j.bbabio.2011.10.006
000020870 0247_ $$2WOS$$aWOS:000300265300013
000020870 037__ $$aPreJuSER-20870
000020870 041__ $$aeng
000020870 082__ $$a570
000020870 084__ $$2WoS$$aBiochemistry & Molecular Biology
000020870 084__ $$2WoS$$aBiophysics
000020870 1001_ $$0P:(DE-Juel1)128970$$aKoch-Koerfges, A.$$b0$$uFZJ
000020870 245__ $$aPhysiology and global gene expression of a Corynebacterium glutamicum ΔF1FO-ATP synthase mutant devoid of oxidative phosphorylation
000020870 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2012
000020870 300__ $$a370 - 380
000020870 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000020870 3367_ $$2DataCite$$aOutput Types/Journal article
000020870 3367_ $$00$$2EndNote$$aJournal Article
000020870 3367_ $$2BibTeX$$aARTICLE
000020870 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000020870 3367_ $$2DRIVER$$aarticle
000020870 440_0 $$0802$$aBiochimica et Biophysica Acta$$v1817$$x0006-3002$$y2
000020870 500__ $$aFinancial support (grant 0315598 to MB and KM) by the Bundesministerium fur Bildung und Forschung (BMBF) is gratefully acknowledged. The authors would like to thank Dr. Melanie Brocker for the help with MALDI-TOF-MS measurements, Brita Weil and Ulrike Viets for excellent technical assistance, and Prof. Reinhard Kramer (Universitat zu Koln) for his support of the measurements of bioenergetic parameters.
000020870 520__ $$aA mutant of Corynebacterium glutamicum ATCC 13032 with a deletion of the atpBEFHAGDC genes encoding F(1)F(O)-ATP synthase was characterized. Whereas no growth was observed with acetate as sole carbon source, the ΔF(1)F(O) mutant reached 47% of the growth rate and 65% of the biomass of the wild type during shake-flask cultivation in glucose minimal medium. Initially, the mutant strain showed a strongly increased glucose uptake rate accompanied by a high oxygen consumption rate and pyruvate secretion into the medium. When oxygen became limiting, the glucose consumption rate was reduced below that of the wild type and pyruvate was consumed again. The ΔF(1)F(O) mutant had increased levels of b- and d-type cytochromes and a significantly increased proton motive force. Transcription of genes involved in central carbon metabolism was essentially unchanged, whereas genes for cytochrome bd oxidase, pyruvate:quinone oxidoreductase, oxidative stress response, and others showed increased mRNA levels. On the other hand, genes for amino acid biosynthesis and ribosomal proteins as well as many genes involved in transport displayed decreased mRNA levels. Several of the transcriptional changes were reflected at the protein level, but there were also discrepancies between the mRNA and protein levels suggesting some kind of posttranscriptional regulation. The results prove for the first time that F(1)F(O)-ATP synthase and oxidative phosphorylation are in general not essential for growth of C. glutamicum.
000020870 536__ $$0G:(DE-Juel1)FUEK410$$2G:(DE-HGF)$$aBiotechnologie$$cPBT$$x0
000020870 588__ $$aDataset connected to Web of Science, Pubmed
000020870 65320 $$2Author$$aF1FO-ATP synthase
000020870 65320 $$2Author$$aOxidative phosphorylation
000020870 65320 $$2Author$$aatpBEFHAGDC deletion
000020870 65320 $$2Author$$aCorynebacterium glutamicum
000020870 65320 $$2Author$$aProton motive force
000020870 65320 $$2Author$$aGene expression
000020870 650_2 $$2MeSH$$aAcids: metabolism
000020870 650_2 $$2MeSH$$aCorynebacterium glutamicum: genetics
000020870 650_2 $$2MeSH$$aCorynebacterium glutamicum: growth & development
000020870 650_2 $$2MeSH$$aCorynebacterium glutamicum: metabolism
000020870 650_2 $$2MeSH$$aCorynebacterium glutamicum: physiology
000020870 650_2 $$2MeSH$$aElectron Transport: drug effects
000020870 650_2 $$2MeSH$$aElectron Transport: genetics
000020870 650_2 $$2MeSH$$aElectron Transport: physiology
000020870 650_2 $$2MeSH$$aGene Expression Profiling
000020870 650_2 $$2MeSH$$aGene Expression Regulation, Bacterial: drug effects
000020870 650_2 $$2MeSH$$aGlucose: metabolism
000020870 650_2 $$2MeSH$$aGlucose: pharmacology
000020870 650_2 $$2MeSH$$aGlycogen: metabolism
000020870 650_2 $$2MeSH$$aOrganisms, Genetically Modified
000020870 650_2 $$2MeSH$$aOxidative Phosphorylation
000020870 650_2 $$2MeSH$$aOxygen Consumption: genetics
000020870 650_2 $$2MeSH$$aOxygen Consumption: physiology
000020870 650_2 $$2MeSH$$aProteome: analysis
000020870 650_2 $$2MeSH$$aProteome: metabolism
000020870 650_2 $$2MeSH$$aProton-Translocating ATPases: genetics
000020870 650_2 $$2MeSH$$aProton-Translocating ATPases: physiology
000020870 650_2 $$2MeSH$$aSequence Deletion
000020870 650_7 $$00$$2NLM Chemicals$$aAcids
000020870 650_7 $$00$$2NLM Chemicals$$aProteome
000020870 650_7 $$050-99-7$$2NLM Chemicals$$aGlucose
000020870 650_7 $$09005-79-2$$2NLM Chemicals$$aGlycogen
000020870 650_7 $$0EC 3.6.3.14$$2NLM Chemicals$$aProton-Translocating ATPases
000020870 650_7 $$2WoSType$$aJ
000020870 7001_ $$0P:(DE-HGF)0$$aKabus, A.$$b1
000020870 7001_ $$0P:(DE-HGF)0$$aOchrombel, I.$$b2
000020870 7001_ $$0P:(DE-HGF)0$$aMarin, K.$$b3
000020870 7001_ $$0P:(DE-Juel1)128943$$aBott, M.$$b4$$uFZJ
000020870 773__ $$0PERI:(DE-600)1460387-1$$a10.1016/j.bbabio.2011.10.006$$gVol. 1817, p. 370 - 380$$p370 - 380$$q1817<370 - 380$$tBiochimica et biophysica acta$$v1817$$x0006-3002$$y2012
000020870 8567_ $$uhttp://dx.doi.org/10.1016/j.bbabio.2011.10.006
000020870 909CO $$ooai:juser.fz-juelich.de:20870$$pVDB
000020870 915__ $$0StatID:(DE-HGF)0010$$2StatID$$aJCR/ISI refereed
000020870 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR
000020870 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000020870 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000020870 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000020870 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000020870 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz
000020870 9141_ $$y2012
000020870 9131_ $$0G:(DE-Juel1)FUEK410$$1G:(DE-HGF)POF2-890$$2G:(DE-HGF)POF2-800$$aDE-HGF$$baußerhalb PoF$$kPBT$$lohne FE$$vBiotechnologie$$x0
000020870 9132_ $$0G:(DE-HGF)POF3-581$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vBiotechnology$$x0
000020870 9201_ $$0I:(DE-Juel1)VDB55$$gIBT$$kIBT-1$$lBiotechnologie 1$$x0$$zab 31.10.10 weitergeführt als IBG-1
000020870 970__ $$aVDB:(DE-Juel1)136613
000020870 980__ $$aVDB
000020870 980__ $$aConvertedRecord
000020870 980__ $$ajournal
000020870 980__ $$aI:(DE-Juel1)IBG-1-20101118
000020870 980__ $$aUNRESTRICTED
000020870 981__ $$aI:(DE-Juel1)IBG-1-20101118