TY  - JOUR
AU  - Koch-Koerfges, A.
AU  - Kabus, A.
AU  - Ochrombel, I.
AU  - Marin, K.
AU  - Bott, M.
TI  - Physiology and global gene expression of a Corynebacterium glutamicum ΔF1FO-ATP synthase mutant devoid of oxidative phosphorylation
JO  - Biochimica et biophysica acta
VL  - 1817
SN  - 0006-3002
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - PreJuSER-20870
SP  - 370 - 380
PY  - 2012
N1  - Financial support (grant 0315598 to MB and KM) by the Bundesministerium fur Bildung und Forschung (BMBF) is gratefully acknowledged. The authors would like to thank Dr. Melanie Brocker for the help with MALDI-TOF-MS measurements, Brita Weil and Ulrike Viets for excellent technical assistance, and Prof. Reinhard Kramer (Universitat zu Koln) for his support of the measurements of bioenergetic parameters.
AB  - A mutant of Corynebacterium glutamicum ATCC 13032 with a deletion of the atpBEFHAGDC genes encoding F(1)F(O)-ATP synthase was characterized. Whereas no growth was observed with acetate as sole carbon source, the ΔF(1)F(O) mutant reached 47% of the growth rate and 65% of the biomass of the wild type during shake-flask cultivation in glucose minimal medium. Initially, the mutant strain showed a strongly increased glucose uptake rate accompanied by a high oxygen consumption rate and pyruvate secretion into the medium. When oxygen became limiting, the glucose consumption rate was reduced below that of the wild type and pyruvate was consumed again. The ΔF(1)F(O) mutant had increased levels of b- and d-type cytochromes and a significantly increased proton motive force. Transcription of genes involved in central carbon metabolism was essentially unchanged, whereas genes for cytochrome bd oxidase, pyruvate:quinone oxidoreductase, oxidative stress response, and others showed increased mRNA levels. On the other hand, genes for amino acid biosynthesis and ribosomal proteins as well as many genes involved in transport displayed decreased mRNA levels. Several of the transcriptional changes were reflected at the protein level, but there were also discrepancies between the mRNA and protein levels suggesting some kind of posttranscriptional regulation. The results prove for the first time that F(1)F(O)-ATP synthase and oxidative phosphorylation are in general not essential for growth of C. glutamicum.
KW  - Acids: metabolism
KW  - Corynebacterium glutamicum: genetics
KW  - Corynebacterium glutamicum: growth & development
KW  - Corynebacterium glutamicum: metabolism
KW  - Corynebacterium glutamicum: physiology
KW  - Electron Transport: drug effects
KW  - Electron Transport: genetics
KW  - Electron Transport: physiology
KW  - Gene Expression Profiling
KW  - Gene Expression Regulation, Bacterial: drug effects
KW  - Glucose: metabolism
KW  - Glucose: pharmacology
KW  - Glycogen: metabolism
KW  - Organisms, Genetically Modified
KW  - Oxidative Phosphorylation
KW  - Oxygen Consumption: genetics
KW  - Oxygen Consumption: physiology
KW  - Proteome: analysis
KW  - Proteome: metabolism
KW  - Proton-Translocating ATPases: genetics
KW  - Proton-Translocating ATPases: physiology
KW  - Sequence Deletion
KW  - Acids (NLM Chemicals)
KW  - Proteome (NLM Chemicals)
KW  - Glucose (NLM Chemicals)
KW  - Glycogen (NLM Chemicals)
KW  - Proton-Translocating ATPases (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:22050934
UR  - <Go to ISI:>//WOS:000300265300013
DO  - DOI:10.1016/j.bbabio.2011.10.006
UR  - https://juser.fz-juelich.de/record/20870
ER  -