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@ARTICLE{KochKoerfges:20870,
      author       = {Koch-Koerfges, A. and Kabus, A. and Ochrombel, I. and
                      Marin, K. and Bott, M.},
      title        = {{P}hysiology and global gene expression of a
                      {C}orynebacterium glutamicum Δ{F}1{FO}-{ATP} synthase
                      mutant devoid of oxidative phosphorylation},
      journal      = {Biochimica et biophysica acta},
      volume       = {1817},
      issn         = {0006-3002},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {PreJuSER-20870},
      pages        = {370 - 380},
      year         = {2012},
      note         = {Financial support (grant 0315598 to MB and KM) by the
                      Bundesministerium fur Bildung und Forschung (BMBF) is
                      gratefully acknowledged. The authors would like to thank Dr.
                      Melanie Brocker for the help with MALDI-TOF-MS measurements,
                      Brita Weil and Ulrike Viets for excellent technical
                      assistance, and Prof. Reinhard Kramer (Universitat zu Koln)
                      for his support of the measurements of bioenergetic
                      parameters.},
      abstract     = {A mutant of Corynebacterium glutamicum ATCC 13032 with a
                      deletion of the atpBEFHAGDC genes encoding F(1)F(O)-ATP
                      synthase was characterized. Whereas no growth was observed
                      with acetate as sole carbon source, the ΔF(1)F(O) mutant
                      reached $47\%$ of the growth rate and $65\%$ of the biomass
                      of the wild type during shake-flask cultivation in glucose
                      minimal medium. Initially, the mutant strain showed a
                      strongly increased glucose uptake rate accompanied by a high
                      oxygen consumption rate and pyruvate secretion into the
                      medium. When oxygen became limiting, the glucose consumption
                      rate was reduced below that of the wild type and pyruvate
                      was consumed again. The ΔF(1)F(O) mutant had increased
                      levels of b- and d-type cytochromes and a significantly
                      increased proton motive force. Transcription of genes
                      involved in central carbon metabolism was essentially
                      unchanged, whereas genes for cytochrome bd oxidase,
                      pyruvate:quinone oxidoreductase, oxidative stress response,
                      and others showed increased mRNA levels. On the other hand,
                      genes for amino acid biosynthesis and ribosomal proteins as
                      well as many genes involved in transport displayed decreased
                      mRNA levels. Several of the transcriptional changes were
                      reflected at the protein level, but there were also
                      discrepancies between the mRNA and protein levels suggesting
                      some kind of posttranscriptional regulation. The results
                      prove for the first time that F(1)F(O)-ATP synthase and
                      oxidative phosphorylation are in general not essential for
                      growth of C. glutamicum.},
      keywords     = {Acids: metabolism / Corynebacterium glutamicum: genetics /
                      Corynebacterium glutamicum: growth $\&$ development /
                      Corynebacterium glutamicum: metabolism / Corynebacterium
                      glutamicum: physiology / Electron Transport: drug effects /
                      Electron Transport: genetics / Electron Transport:
                      physiology / Gene Expression Profiling / Gene Expression
                      Regulation, Bacterial: drug effects / Glucose: metabolism /
                      Glucose: pharmacology / Glycogen: metabolism / Organisms,
                      Genetically Modified / Oxidative Phosphorylation / Oxygen
                      Consumption: genetics / Oxygen Consumption: physiology /
                      Proteome: analysis / Proteome: metabolism /
                      Proton-Translocating ATPases: genetics /
                      Proton-Translocating ATPases: physiology / Sequence Deletion
                      / Acids (NLM Chemicals) / Proteome (NLM Chemicals) / Glucose
                      (NLM Chemicals) / Glycogen (NLM Chemicals) /
                      Proton-Translocating ATPases (NLM Chemicals) / J (WoSType)},
      cin          = {IBT-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB55},
      pnm          = {Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK410},
      shelfmark    = {Biochemistry $\&$ Molecular Biology / Biophysics},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:22050934},
      UT           = {WOS:000300265300013},
      doi          = {10.1016/j.bbabio.2011.10.006},
      url          = {https://juser.fz-juelich.de/record/20870},
}